| Diaphragm pico-liter pump for single-cell manipulation. | |
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MedLine Citation:
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PMID: 21494795 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A pico-liter pump is developed and integrated into a robotic manipulation system that automatically selects and transfers individual living cells of interest to analysis locations. The pump is a displacement type pump comprising one cylindrical chamber connected to a capillary micropipette. The top of the chamber is a thin diaphragm which, when deflected, causes the volume of the fluid-filled cylindrical chamber to change thereby causing fluid in the chamber to flow in and out of the micropipette. This enables aspirating and dispensing individual living cells. The diaphragm is deflected by a piezoelectric actuator that pushes against its center. The pump aspirates and dispenses volumes of fluid between 500 pL and 250 nL at flow rates up to 250 nL/s. The piezo-driven diaphragm arrangement provides exquisite control of the flow rate in and out of the capillary orifice. This feature, in turn, allows reduced perturbation of live cells by controlling and minimizing the applied shear stresses. |
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Authors:
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Yasser Anis; Jeffrey Houkal; Mark Holl; Roger Johnson; Deirdre Meldrum |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-15 |
Journal Detail:
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Title: Biomedical microdevices Volume: - ISSN: 1572-8781 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100887374 Medline TA: Biomed Microdevices Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Mechanical Design and Production Engineering, Cairo University, Giza, 12316, Egypt, yasser.anis@ieee.org. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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