Document Detail

The Diaphanous-related Formins Promote Protrusion Formation and Cell-to-Cell Spread of Listeria monocytogenes.
MedLine Citation:
PMID:  25281757     Owner:  NLM     Status:  Publisher    
The Gram-positive bacterium Listeria monocytogenes is a facultative intracellular pathogen whose virulence depends on its ability to spread from cell-to-cell within an infected host. Although Arp2/3 is necessary and sufficient for Listeria actin tail assembly, previous studies suggest that other actin polymerization factors, such as formins, may participate in protrusion formation. Here, we show that Arp2/3 localized to only a minor portion of the protrusion. Moreover, treatment of L. monocytogenes infected HeLa cells with a formin FH2-domain inhibitor significantly reduced protrusion length. In addition, the Diaphanous-related formins (mDia)1-3 localized to protrusions and knockdown of mDia1, 2, and 3 substantially decreased cell-to-cell spread of L. monocytogenes. Rho GTPases are known to be involved in formin activation. Our studies also show that knockdown of several Rho family members significantly impacted bacterial cell-to-cell spread. Collectively, these findings identify a Rho GTPase-formin network that is critically involved in the cell-to-cell spread of L. monocytogenes.
Ramzi Fattouh; Mark A Czuczman; Hyunwoo Kwon; John W Copeland; Laurence Pelletier; Margot Quinlan; Aleixo M Muise; Darren E Higgins; John H Brumell
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-10-3
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  -     ISSN:  1537-6613     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  2014 Oct 
Date Detail:
Created Date:  2014-10-4     Completed Date:  -     Revised Date:  2014-10-5    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail:
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