| Diallyl trisulfide suppresses the adipogenesis of 3T3-L1 preadipocytes through ERK activation. | |
| | |
MedLine Citation:
|
PMID: 22137902 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Garlic and its organosulfur compounds display hypolipidemic effects by inhibiting fatty acid and cholesterol synthesis. We investigated the anti-adipogenic effect of diallyl trisulfide (DATS), the second most abundant organosulfide in garlic oil. We studied the effects of DATS in 3T3-L1 preadipocytes and the mechanism involved in its action. DATS (0-75μM) inhibited CCAAT/enhancer-binding protein (C/EBP) α and β and peroxisome proliferator-activated receptor (PPAR) γ mRNA and protein levels in a dose- and time-dependent manner, leading to a decrease of fatty acid synthase (FAS) expression and lipid accumulation in 3T3-L1 cells. Insulin treatment induced a transient increase in ERK phosphorylation in 3T3-L1 preadipocytes, and maximal induction was observed at 5min and then declined. DATS, however, sustained ERK phosphorylation up to 120min. In the presence of PD98059, the phosphorylation of ERK and suppression of PPARγ expression and DNA binding activity of PPARγ by DATS were reversed, and lipid accumulation was restored. In conclusion, these results indicate that DATS inhibits the differentiation of 3T3-L1 preadipocytes into adipocytes. DATS is likely to act by prolonging ERK activation, which leads to the down-regulation of adipogenic transcription factor expression during adipogenesis. Our results suggest that garlic may have potential as an anti-obesity agent. |
| | |
Authors:
|
Chong-Kuei Lii; Chun-Yin Huang; Haw-Wen Chen; Mun-Yew Chow; Yu-Ru Lin; Chin-Shiu Huang; Chia-Wen Tsai |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-11-25 |
Journal Detail:
|
Title: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association Volume: - ISSN: 1873-6351 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-12-5 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8207483 Medline TA: Food Chem Toxicol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier Ltd. |
Affiliation:
|
Department of Nutrition, China Medical University, No. 91, Hsueh-Shih Rd., Taichung 404, Taiwan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: In vitro genotoxicity evaluation of 4-carboxyl-2,6-dinitrophenylazohydroxynaphthalenes using human l...
Next Document: Triazine residues in raw milk and infant formulas from Spanish northwest, by a diphasic dialysis ext...