Document Detail


Diallyl trisulfide as an inhibitor of benzo(a)pyrene-induced precancerous carcinogenesis in MCF-10A cells.
MedLine Citation:
PMID:  22525868     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Diallyl trisulfide (DATS) is a garlic organosulfide that is toxic to cancer cells, however, little is known about its effect in the initiation phase of carcinogenesis. We sought to determine whether DATS could inhibit the carcinogen, benzo(a)pyrene (BaP), from inducing precancerous activity, in vitro. MCF-10A cells were either pre-treated (PreTx) or concurrently treated (CoTx) with 1 μM BaP, and 6 or 60 μM DATS for up to 24h. The DATS 6 and 60 μM CoTx inhibited BaP-induced cell proliferation by an average of 71.1% and 120.8%, respectively, at 6h. The 60 μM DATS pretreatment decreased BaP-induced G2/M cell cycle transition by 127%, and reduced the increase in cells in the S-phase by 42%; whereas 60 μM DATS CoTx induced a 177% increase in cells in G1. DATS effectively inhibited (P<0.001) BaP-induced peroxide formation by at least 54%, which may have prevented the formation of BaP-induced DNA strand breaks. In this study, we reveal mechanisms involved in DATS inhibition of BaP-induced carcinogenesis, including inhibition of cell proliferation, regulation of cell cycle, attenuation of ROS formation, and inhibition of DNA damage. At the doses evaluated, DATS appears to be an effective attenuator of BaP-induced breast carcinogenesis, in vitro.
Authors:
Yasmeen M Nkrumah-Elie; Jayne S Reuben; Alicia Hudson; Equar Taka; Ramesh Badisa; Tiffany Ardley; Bridg'ette Israel; Sakeenah Y Sadrud-Din; Ebenezer Oriaku; Selina F Darling-Reed
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.     Date:  2012-04-16
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  50     ISSN:  1873-6351     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-13     Completed Date:  2012-10-12     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2524-30     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Allyl Compounds / pharmacology*
Benzo(a)pyrene / antagonists & inhibitors*,  toxicity
Cell Line, Tumor
Cell Transformation, Neoplastic*
Comet Assay
DNA Damage
Flow Cytometry
Humans
Sulfides / pharmacology*
Grant Support
ID/Acronym/Agency:
2 G12 RR003020/RR/NCRR NIH HHS; 8 G12 MD007582-28/MD/NIMHD NIH HHS; G12 MD007582/MD/NIMHD NIH HHS; G12 RR003020/RR/NCRR NIH HHS; G12 RR003020-27/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Allyl Compounds; 0/Sulfides; 0ZO1U5A3XX/diallyl trisulfide; 3417WMA06D/Benzo(a)pyrene
Comments/Corrections

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