| Diagnostic value of p16INK4A, Ki-67, and human papillomavirus L1 capsid protein immunochemical staining on cell blocks from residual liquid-based gynecologic cytology specimens. | |
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MedLine Citation:
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PMID: 20069634 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: This study was conducted to evaluate the reliability and role of cell block preparations in the diagnosis of neoplastic and preneoplastic lesions of the cervix and to improve the value of cell block preparations in diagnosing and predicting the prognosis of cervical lesions through immunostaining of p16INK4A (p16), Ki-67, and human papillomavirus (HPV) L1 capsid protein (HPV L1). METHODS: In total, 138 specimens were diagnosed on liquid-based cytology (LBC) and cell block preparations, and 63 specimens were subjected subsequently to tissue follow-up and immunostaining for p16, Ki-67, and HPV L1 on cell block sections. RESULTS: In 42 specimens that were diagnosed as low-grade squamous intraepithelial lesion, high-grade squamous intraepithelial lesion (HSIL), and squamous cell carcinoma (SCC) on cell blocks, 38 specimens (90.5%) were confirmed by histopathologic reports, and there was slightly better than 81.6% agreement between LBC and tissue follow-up. Immunointensity and cells that were positive for p16 were enhanced according to increased pathologic grade and differed statistically between cervical intraepithelial neoplasia 1 (CIN-1) and CIN-2/CIN-3 as well as SCC. The positive rates of HPV L1 decreased gradually according to the severity of cervical neoplasia, and HPV L1/p16 expression patterns were related to the severity of cervical lesions. CONCLUSIONS: The cell block preparation technique was complementary to LBC, and the authors concluded that the application of LBC combined with cell block preparations may improve the diagnostic accuracy of cytology. Immunostaining for p16 and Ki-67 on cell block preparations can help to improve the diagnostic accuracy of HSIL and SCC. A combined expression pattern of p16 and HPV L1 may serve as a valuable index for predicting prognosis and follow-up of cervical dysplastic lesions. |
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Authors:
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Li Yu; Liantang Wang; Juemin Zhong; Shangwu Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer cytopathology Volume: 118 ISSN: 1934-662X ISO Abbreviation: Cancer Cytopathol Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-26 Completed Date: 2010-04-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101499453 Medline TA: Cancer Cytopathol Country: United States |
Other Details:
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Languages: eng Pagination: 47-55 Citation Subset: IM |
Copyright Information:
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(c) 2010 American Cancer Society. |
Affiliation:
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Department of Histopathology, First Affiliated Hospital, Sun Yat-sen (Zhongshan) University, Guangzhou, People's Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Aged, 80 and over Capsid Proteins / metabolism* Carcinoma, Squamous Cell / diagnosis*, metabolism Cervical Intraepithelial Neoplasia / diagnosis*, metabolism Cyclin-Dependent Kinase Inhibitor p16 / metabolism* Cytodiagnosis / methods* Female Follow-Up Studies Humans Immunoenzyme Techniques Ki-67 Antigen / metabolism* Middle Aged Oncogene Proteins, Viral / metabolism* Precancerous Conditions / diagnosis*, metabolism Prognosis Tumor Markers, Biological / analysis Uterine Cervical Neoplasms / diagnosis*, metabolism Vaginal Smears Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Capsid Proteins; 0/Cyclin-Dependent Kinase Inhibitor p16; 0/HPV L1 protein, Human papillomavirus; 0/Ki-67 Antigen; 0/Oncogene Proteins, Viral; 0/Tumor Markers, Biological |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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