Document Detail


Diagnostic value of HCMV pp65 antigen detection by FCA for symptomatic and asymptomatic infection: compared to quantification of HCMV DNA and detection of IgM antibody in infants.
MedLine Citation:
PMID:  19308446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human cytomegalovirus (HCMV) can cause symptomatic or asymptomatic infection in infants. One hundred and twenty-six infants were assessed clinically for disease in infantile period. Eighty of them were classified as symptomatic infection on the basis of physical, instrumental, and laboratory findings, 5 were demonstrated by following up to have later developed HCMV disease, and the other 41 infants were classified as asymptomatic infection. HCMV DNA was positive in all urine samples of the symptomatic infants detected by quantitative polymerase chain reaction. HCMV-IgM antibody detected by chemiluminescent immunoassay (CLIA) was positive in 62 of the 85 symptomatic infants, but was negative in all of the samples of asymptomatic infants. HCMV pp65 antigen detected by flow cytometry assay (FCA) was positive in 77 of the 85 symptomatic infants and in none of the asymptomatic infants. The coincidence to symptom of HCMV pp65 antigen detection was higher than those of HCMV DNA and HCMV-IgM antibody detection. The sensitivity, specificity, positive prognostic value and the negative prognostic value of HCMV pp65 antigen detection for diagnosis of HCMV infection was 90.6, 100, 100 and 83.7%, respectively. We concluded that detection of pp65 antigen by FCA is more sensitive for diagnosis of HCMV infection than detection of HCMV-IgM antibody and is better than HCMV DNA quantification for distinguishing the symptomatic and asymptomatic HCMV infection in infants.
Authors:
Zhengrong Sun; Xiangshi Ceng; Zhiqin Mao; Jidong Wang; Qing Liu; Yaohua Ji; Yanping Ma; Ying Qi; Rong He; Qiang Ruan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-24
Journal Detail:
Title:  Medical microbiology and immunology     Volume:  198     ISSN:  1432-1831     ISO Abbreviation:  Med. Microbiol. Immunol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-21     Completed Date:  2009-05-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0314524     Medline TA:  Med Microbiol Immunol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  107-12     Citation Subset:  IM    
Affiliation:
Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, 110004 Shenyang, Liaoning, China.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Viral / diagnostic use*,  immunology,  isolation & purification
Antigens, Viral / diagnostic use
Cytomegalovirus / immunology
Cytomegalovirus Infections / diagnosis*,  immunology
DNA, Viral / isolation & purification*,  urine
Flow Cytometry
Humans
Immunoglobulin M / diagnostic use*,  immunology,  isolation & purification
Infant
Phosphoproteins / diagnostic use*
Predictive Value of Tests
Viral Matrix Proteins / diagnostic use*
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/Antigens, Viral; 0/DNA, Viral; 0/Immunoglobulin M; 0/Phosphoproteins; 0/Viral Matrix Proteins; 0/cytomegalovirus matrix protein 65kDa

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