| Diagnostic utility of flow cytometry in low-grade myelodysplastic syndromes: a prospective validation study. | |
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MedLine Citation:
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PMID: 19546439 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The diagnosis of myelodysplastic syndromes is not always straightforward when patients lack specific diagnostic markers, such as blast excess, karyotype abnormality, and ringed sideroblasts. DESIGN AND METHODS: We designed a flow cytometry protocol applicable in many laboratories and verified its diagnostic utility in patients without those diagnostic markers. The cardinal parameters, analyzable from one cell aliquot, were myeloblasts (%), B-cell progenitors (%), myeloblast CD45 expression, and channel number of side scatter where the maximum number of granulocytes occurs. The adjunctive parameters were CD11b, CD15, and CD56 expression (%) on myeloblasts. Marrow samples from 106 control patients with cytopenia and 134 low-grade myelodysplastic syndromes patients, including 81 lacking both ringed sideroblasts and cytogenetic aberrations, were prospectively analyzed in Japan and Italy. RESULTS: Data outside the predetermined reference range in 2 or more parameters (multiple abnormalities) were common in myelodysplastic syndromes patients. In those lacking ringed sideroblasts and cytogenetic aberrations, multiple abnormalities were observed in 8/26 Japanese (30.8%) and 37/55 Italians (67.3%) when the cardinal parameters alone were considered, and in 17/26 Japanese (65.4%) and 42/47 Italians (89.4%) when all parameters were taken into account. Multiple abnormalities were rare in controls. When data from all parameters were used, the diagnostic sensitivities were 65% and 89%, specificities were 98% and 90%, and likelihood ratios were 28.1 and 8.5 for the Japanese and Italian cohorts, respectively. CONCLUSIONS: This protocol can be used in the diagnostic work-up of low-grade myelodysplastic syndromes patients who lack specific diagnostic markers, although further improvement in diagnostic power is desirable. |
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Authors:
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Kiyoyuki Ogata; Matteo G Della Porta; Luca Malcovati; Cristina Picone; Norio Yokose; Akira Matsuda; Taishi Yamashita; Hideto Tamura; Junichi Tsukada; Kazuo Dan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies Date: 2009-06-22 |
Journal Detail:
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Title: Haematologica Volume: 94 ISSN: 1592-8721 ISO Abbreviation: Haematologica Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-31 Completed Date: 2009-12-07 Revised Date: 2010-09-27 |
Medline Journal Info:
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Nlm Unique ID: 0417435 Medline TA: Haematologica Country: Italy |
Other Details:
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Languages: eng Pagination: 1066-74 Citation Subset: IM |
Affiliation:
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Division of Hematology, Department of Medicine, Nippon Medical School, Tokyo, Japan. ogata@nms.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Antigens, CD11b / analysis Antigens, CD15 / analysis Antigens, CD45 / analysis Antigens, CD56 / analysis Bone Marrow Cells / metabolism Female Flow Cytometry / methods* Humans Immunophenotyping / methods Male Middle Aged Myelodysplastic Syndromes / blood, diagnosis*, immunology Prospective Studies Reproducibility of Results Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD11b; 0/Antigens, CD15; 0/Antigens, CD56; EC 3.1.3.48/Antigens, CD45 |
| Comments/Corrections | |
Comment In:
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Haematologica. 2009 Aug;94(8):1041-3
[PMID:
19644135
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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