Document Detail


Diagnostic patterns of very-long-chain fatty acids in plasma of patients with X-linked adrenoleukodystrophy.
MedLine Citation:
PMID:  11522057     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pattern recognition analysis on the levels of the very-long-chain fatty acids (VLCFAs) in plasma is described for the visual discrimination of X-linked adrenoleukodystrophy (X-ALD) patients from normal healthy group. Plasma VLCFA compositions of 58 normal subjects and 16 X-ALD patients were examined by gas chromatography as their methyl esters to determine the area percentages of behenic acid (C22:0), lignoceric acid (C24:0) and hexacosanoic acid (C26:0) in the total fatty acids, and the concentration (microg/ml) of C26:0. When star symbol plotting was applied to the VLCFA values of C22:0 (%), C24:0 (%), C26:0 (%), C24:0/C22:0, C26:0/C22:0 and C26:0 (microg/ml) after normalization to the corresponding median values in normal group, the resulting deformed hexagonal star pattern was characteristic of each patient. Therefore, simple visual comparison with the equilateral hexagon of normal group average as the control pattern enabled one readily to discriminate X-ALD patients from the normal group. Additionally, canonical discriminant analysis performed on the six unnormalized VLCFA values correctly classified 74 plasma specimens into two separate clusters according to normal subject or X-ALD patient in the canonical plot.
Authors:
M J Paik; K R Kim; H R Yoon; H J Kim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical sciences and applications     Volume:  760     ISSN:  1387-2273     ISO Abbreviation:  J. Chromatogr. B Biomed. Sci. Appl.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-08-27     Completed Date:  2002-02-05     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9714109     Medline TA:  J Chromatogr B Biomed Sci Appl     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  149-57     Citation Subset:  IM    
Affiliation:
Seoul Clinical Laboratories (SCL), Seoul Medical Science Institute, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Adrenoleukodystrophy / blood,  diagnosis*,  genetics
Case-Control Studies
Chromatography, Gas
Fatty Acids / blood*
Humans
Linkage (Genetics)*
X Chromosome*
Chemical
Reg. No./Substance:
0/Fatty Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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