| Diagnostic microarray for human and animal bacterial diseases and their virulence and antimicrobial resistance genes. | |
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MedLine Citation:
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PMID: 20035807 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rapid diagnosis and treatment of disease is often based on the identification and characterization of causative agents derived from phenotypic characteristics. Current methods can be laborious and time-consuming, often requiring many skilled personnel and a large amount of lab space. The objective of our study was to develop a spotted microarray for rapid identification and characterization of bacterial pathogens and their antimicrobial resistance genes. Our spotted microarray consists of 489 70mer probes that detect 40 bacterial pathogens of medical, veterinary and zoonotic importance (including 15 NIAID Category A, B and C pathogens); associated genes that encode resistance for antimicrobial and metal resistance; and DNA elements that are important for horizontal gene transfer among bacteria. High specificity and reliability of the microarray was achieved for bacterial pathogens of animal and human importance by validating MDR pathogenic bacteria as pure cultures or by following their inoculation in complex and highly organic sample matrices, such as soil and manure. |
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Authors:
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Greg Peterson; Jianfa Bai; T G Nagaraja; Sanjeev Narayanan |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-12-24 |
Journal Detail:
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Title: Journal of microbiological methods Volume: 80 ISSN: 1872-8359 ISO Abbreviation: J. Microbiol. Methods Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-22 Completed Date: 2010-05-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8306883 Medline TA: J Microbiol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 223-30 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Diagnostic Medicine/Pathobiology, Kansas State University, College of Veterinary Medicine, Manhattan, KS 66502, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle DNA, Bacterial / genetics, isolation & purification Drug Resistance, Bacterial / genetics Enterococcus faecalis / genetics*, isolation & purification*, pathogenicity Enterococcus faecium / genetics*, isolation & purification*, pathogenicity Escherichia coli / genetics, isolation & purification, pathogenicity Feces / microbiology Fusobacterium necrophorum / genetics, isolation & purification, pathogenicity Genes, Bacterial Genes, MDR* Gram-Negative Bacteria / genetics*, isolation & purification*, pathogenicity Gram-Negative Bacterial Infections / diagnosis*, microbiology Gram-Positive Bacterial Infections / diagnosis*, microbiology Humans Oligonucleotide Array Sequence Analysis / methods* Salmonella typhimurium / genetics, isolation & purification, pathogenicity Sensitivity and Specificity Soil Microbiology Species Specificity Virulence Virulence Factors / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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P20 RR016469/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/Virulence Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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