Document Detail

[Diagnostic assessment of peritoneal fluid cytology in horses with abdominal neoplasia].
MedLine Citation:
PMID:  22526721     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate the diagnostic value of peritoneal fluid (PF) cytology for clinical diagnosis of abdominal neoplasia in horses.
MATERIAL AND METHODS: Ten horses with histopathologically confirmed abdominal neoplasia, in which a PF analysis was performed, were included in this retrospective study. PF was analyzed for total protein concentration and a nucleated cell count was performed. Using cytological criteria of malignancy, the PF samples were evaluated regarding their probability of malignancy.
RESULTS: Cytologic classification of cells according to criteria of malignancy allowed a positive cytologic diagnosis of neoplasia in 5 out of 10 peritoneal fluid samples. Malignant lymphoma was the most commonly diagnosed neoplasia (3/10) and could be identified by cytology in 2/3 cases. In 1/2 horses with plasma cell myeloma neoplastic cells were similarly found. Malignant melanoma (2/10) was diagnosed using cytology in one case (presence of melanin-containing cells). Cytological diagnosis of malignant neoplasia was established in the only horse with gastric squamous cell carcinoma, but the morphology of the identified tumour cells did not allow a specific diagnosis. Thus, a definitive diagnosis was achieved in 4/5 horses with proven abdominal neoplasia. The horses with adenocarcinoma (1/10) and haemangiosarcoma (1/10) had no evidence of neoplasia based on cytological findings. No relationship between total protein concentration or the nucleated cell count with the histolopathological diagnosis of abdominal neoplasia was found. Abnormal mitotic figures were considered of greater diagnostic value than the overall mitotic rate.
CONCLUSION: The implementation of nuclear criteria of malignancy in the cytologic evaluation of PF samples allows the identification of neoplastic cells to an acceptable degree. For this purpose, the knowledge of the highly variable morphological features of mesothelial cells is essential. The absence of malignant cells does not rule out abdominal neoplasia.
CLINICAL RELEVANCE: PF cytology should be considered as a valuable, minimally invasive, simple, and rapid diagnostic technique in horses with suspected abdominal neoplasia.
S Recknagel; M Nicke; G F Schusser
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere     Volume:  40     ISSN:  1434-1220     ISO Abbreviation:  Tierarztl Prax Ausg G Grosstiere Nutztiere     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-24     Completed Date:  2013-08-26     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  9715779     Medline TA:  Tierarztl Prax Ausg G Grosstiere Nutztiere     Country:  Germany    
Other Details:
Languages:  ger     Pagination:  85-93     Citation Subset:  IM    
Vernacular Title:
Diagnostische Aussagekraft der Zytologie von Bauchpunktaten bei abdominalen Tumoren des Pferdes.
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MeSH Terms
Abdominal Neoplasms / diagnosis,  veterinary*
Adenocarcinoma / diagnosis,  veterinary
Ascitic Fluid / cytology*
Carcinoma, Squamous Cell / diagnosis,  veterinary
Duodenal Neoplasms / diagnosis,  veterinary
Hemangiosarcoma / diagnosis,  veterinary
Horse Diseases / diagnosis*
Lymphoma / diagnosis,  veterinary
Melanoma / diagnosis,  veterinary
Multiple Myeloma / diagnosis,  veterinary
Peritoneal Neoplasms / diagnosis,  veterinary
Stomach Neoplasms / diagnosis,  veterinary

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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