Document Detail

Diagnostic assays for anti-PM/Scl IgG antibodies: heterogeneity in antibody response or lack of standardization?
MedLine Citation:
PMID:  21371448     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The aim of this study was to compare the correlation between new diagnostic methodologies for detecting anti-polymyositis/scleroderma (anti-PM/Scl) IgG antibodies associated with myositis and/or systemic scleroderma assays with existing platforms.
METHODS: Sera from 164 samples previously tested for anti-PM/Scl IgG antibody by immunodiffusion, ID; 171 sera screened for anti-PM/Scl IgG by immunoprecipitation, IP; an additional group of 215 sera tested by ID and 46 healthy blood donor sera were retrospectively evaluated. Anti-PM/Scl IgG antibodies were measured using three PM/Scl-100 specific enzyme immunoassays (EIAs), PM1-alpha (PM1-α) EIA and a line immunoblot assay (LIA) for anti-PM/Scl-75 and -100 IgG antibodies. Selected samples were tested for the presence of antinuclear antibody (ANA) by indirect fluorescent antibody (IFA) assay.
RESULTS: The overall agreement between ID and all anti-PM/Scl IgG EIAs as determined by Crohnbach's alpha was unacceptable (α<0.50). The concordance between the IP and either LIA or PM1-α EIA was greater than 90% however, the best agreement was seen between the IP and LIA PM/Scl-100 assays (98.3%). Compared to the LIA PM/Scl-75 and PM1-α tests, the LIA PM/Scl-100 IgG assay showed the best specificity in the healthy control group.
CONCLUSIONS: Our results demonstrate considerable differences between assays for detecting anti-PM/Scl IgG antibodies which cannot be attributable to heterogeneity in antibody response alone. Further characterization and standardization of these assays are needed.
Troy D Jaskowski; Andrew Wilson; Harry R Hill; Anne E Tebo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-03-01
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  412     ISSN:  1873-3492     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-25     Completed Date:  2012-08-08     Revised Date:  2012-08-14    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1100-5     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Associated Regional and University Pathologists (ARUP) Institute for Clinical and Experimental Pathology, University of Utah School of Medicine, Salt Lake City, UT 84108-1221, United States.
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MeSH Terms
Autoantigens / immunology*
Blood Chemical Analysis / methods*,  standards*
Epitopes / immunology
Immunoglobulin G / blood*,  immunology*
Immunologic Techniques / methods*,  standards*
Reference Standards
Reproducibility of Results
Reg. No./Substance:
0/Autoantigens; 0/Epitopes; 0/Immunoglobulin G; EC 3.1.13.-/EXOSC10 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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