| Diagnostic accuracy of noninvasive polymerase chain reaction testing for the determination of fetal rhesus C, c and E status in early pregnancy. | |
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MedLine Citation:
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PMID: 20175873 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The aim of the study was to determine the sensitivity, specificity and accuracy of noninvasive tests for the fetal rhesus CcEc (RHCE) alleles C, c and E in early pregnancy. DESIGN: A prospective clinical trial was carried out to evaluate diagnostic accuracy. SETTING: Women were recruited at four centres specialising in prenatal diagnosis. Peripheral blood and amniotic fluid samples were obtained and sent to a single laboratory for analysis. SAMPLE: A total of 233 tests (46 for C, 87 for c and 100 for E) were performed on 181 specimens obtained from pregnant women at weeks 12 to 28 (median week 16) of gestation. METHODS: Following automated extraction of fetal DNA from maternal plasma, two different real-time polymerase chain reaction (PCR) protocols were used for the detection of the C, c and E alleles of RHCE. The results of the PCR were compared with genotyping results for the amniotic fluid. MAIN OUTCOME MEASURES: Failure rate, sensitivity, specificity and accuracy were the main outcome measures. RESULTS: Unequivocal results were obtained for all specimens. With the first PCR protocol, the sensitivity was 100% for C, 38% for c and 59% for E. In contrast, with the second protocol, the sensitivity for C, c and E was 100%. The specificity for all assays was found to be between 99% and 100%. CONCLUSIONS: A highly accurate protocol has been identified for the detection of fetal RHCE alleles in maternal plasma in early pregnancy. This noninvasive approach can be considered as a useful test in the management of pregnancies with anti-c, anti-E or anti-C alloimmunisation. |
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Authors:
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K Gutensohn; S P M?ller; K Thomann; W Stein; A Suren; S K?rtge-Jung; G Schl?ter; T J Legler |
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Publication Detail:
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Type: Clinical Trial; Comparative Study; Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't Date: 2010-02-22 |
Journal Detail:
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Title: BJOG : an international journal of obstetrics and gynaecology Volume: 117 ISSN: 1471-0528 ISO Abbreviation: BJOG Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-08 Completed Date: 2010-06-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100935741 Medline TA: BJOG Country: England |
Other Details:
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Languages: eng Pagination: 722-9 Citation Subset: AIM; IM |
Affiliation:
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Department of Transfusion Medicine, University Hospital, Hamburg, Germany. email@kai-gutensohn.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Female Genetic Markers / genetics Genotype Humans Middle Aged Phenotype Polymerase Chain Reaction / methods*, standards Pregnancy Pregnancy Trimester, First Pregnancy Trimester, Second Prenatal Diagnosis / methods*, standards Rh Isoimmunization / diagnosis*, embryology Rh-Hr Blood-Group System / genetics* Sensitivity and Specificity Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Genetic Markers; 0/RHCE protein, human; 0/Rh-Hr Blood-Group System |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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