Document Detail


Diagnostic accuracy of induced sputum LAM ELISA for tuberculosis diagnosis in sputum-scarce patients.
MedLine Citation:
PMID:  22710609     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVE: To examine whether a lipoarabinomannan (LAM) enzyme-linked immunosorbent assay (ELISA) that offers diagnostic utility using urine in patients with tuberculosis (TB) and human immunodeficiency virus (HIV) co-infection can be used in induced sputum to diagnose sputum-scarce patients with suspected TB.DESIGN: LAM was measured in induced sputum samples obtained from 61 consecutively recruited sputum-scarce TB suspects in a tertiary hospital respiratory clinic in South Africa. Liquid culture positivity for Mycobacterium tuberculosis was used as the reference standard. Receiver operating characteristic analysis was used to assess alternative LAM concentration cut-offs.RESULTS: A total of 87% (53/61) of study patients had a valid M. tuberculosis culture result; 49% (23/53) were HIV-infected and 17% (9/53) were culture-positive for M. tuberculosis. Induced sputum smear microscopy and LAM ELISA had an overall sensitivity of 56% (95%CI 27-81); however, the specificity of LAM ELISA was 48% (95%CI 34-62), while the positive and negative predictive values were respectively 18% (95%CI 8-36) and 84% (95%CI 65-94). An optimal rule-in cut-off selected by receiver operating characteristic (LAM concentration >5.73 ng/ml) increased test specificity to 98% and reduced sensitivity to 22%. Normalisation of the assay for sample total protein or cell count did not improve diagnostic accuracy.CONCLUSIONS: In this proof-of-concept study, the ELISA was not clinically useful for TB diagnosis using induced sputum.
Authors:
J G Peter; T J Cashmore; R Meldau; G Theron; R van Zyl-Smit; K Dheda
Related Documents :
22876739 - Testing a service integration model: results from the hiv/aids initiative, connecthiv.
22839749 - Acute hiv-1 infection in the southeastern united states: a cohort study.
1677709 - Diagnosis of tuberculosis by dna amplification in clinical practice evaluation.
22790849 - Exploring the psychological impact of hiv: health comparisons of older australian hiv-p...
22734949 - Meta-analysis of gender differences in efficacy outcomes for hiv-positive subjects in r...
25174279 - Contact dermatitis-like cutaneous leishmaniasis in a libyan hiv patient.
18424739 - Neisseria gonorrhoeae-induced human defensins 5 and 6 increase hiv infectivity: role in...
16048869 - Malawi women and hiv: socio-cultural factors and barriers to prevention.
8567099 - Hepatitis c virus infection in dialysis and clinical nephrology.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-14
Journal Detail:
Title:  The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease     Volume:  -     ISSN:  1815-7920     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706389     Medline TA:  Int J Tuberc Lung Dis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Thermally tunable catalytic and optical properties of gold-hydrogel nanocomposites.
Next Document:  Two Minutes of Sprint Interval Exercise Elicits Similar 24 hour VO2 as 30 Minutes of Continuous Endu...