Document Detail

Diagnostic accuracy of imprint cytology in the assessment of Hodgkin's disease in Japan.
MedLine Citation:
PMID:  15945083     Owner:  NLM     Status:  MEDLINE    
Our objective was to evaluate the usefulness of cytomorphologic assessment in the accuracy of diagnosis of Hodgkin's disease (HD), using imprint cytological preparations over a 18-yr period. Imprint materials from 34 HD cases were reviewed using cytomorphological and immunocytochemical studies. Twenty-six cases (76.5%) were diagnosed to be HD and 6 cases (17.6%) were suspected to be HD, but 2 cases (5.9%) were cytologically diagnosed as reactive lesions, because of an insufficient number of Reed-Sternberg (RS) cells. The 6 suspected cases were definitively diagnosed as HD, using immunocytochemistry. Immunophenotyping of RS cells in 32 cases (excluding the two cases of reactive lesions) showed CD30+ in 31 (96.9%) cases, CD15+ in 22 (68.8%) cases and CD20+ in 12 (37.5%) cases. RS cells were immunophenotypically classified into five groups: A, (CD 30+, 15+, 20-) 15 (46.9%); B, (CD30+, 15-, 20-) 5 (15.6%); C, (CD 30+, 15+, 20+) 6 (18.8%); D, (CD30+, 15-, 20+) 5 (15.6%); and E, (CD30-, 15+, 20+) 1 (3.1%). Cytomorphologic differences in RS cells were identified between group D and other groups (CD15+ and/ or CD20-). The former had a low polymorphic shape (like popcorn), and the latter had a more classical polymorphic shape. Epstein-Barr virus (EBV)-latent membrane protein-1(LMP-1) was identified in 16 (50%) cases. LMP-1 expression was found not only in classic RS cells, but also in smaller variants. These variants did not match the morphologic criteria of RS cells, but expressed the common phenotype (CD30+, CD15+/-) of RS cells, suggesting the same cellular origin as RS cells. This study demonstrated that imprint cytology from lymph node biopsies can be a useful tool for the diagnosis and the evaluation of the cellular biology of HD.
Yasunobu Funamoto; Masami Nagai; Reiji Haba; Masashi Ishikawa; Fujio Kishida; Kohoji Kohno; Toru Matsunaga; Yoshio Kushida; Shoji Kobayashi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Diagnostic cytopathology     Volume:  33     ISSN:  8755-1039     ISO Abbreviation:  Diagn. Cytopathol.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-06-15     Completed Date:  2005-08-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8506895     Medline TA:  Diagn Cytopathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  20-5     Citation Subset:  IM    
Copyright Information:
2005 Wiley-Liss, Inc
Department of Diagnostic Pathology, Kagawa University Hospital, Kita-Gun, Kagawa, Japan.
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MeSH Terms
Aged, 80 and over
Antigens, CD15 / analysis
Antigens, CD20 / analysis
Antigens, CD30 / analysis
Cytodiagnosis / methods*
Hodgkin Disease / diagnosis*,  metabolism
Middle Aged
Reed-Sternberg Cells / chemistry,  pathology
Reproducibility of Results
Sensitivity and Specificity
Viral Matrix Proteins / analysis
Reg. No./Substance:
0/Antigens, CD15; 0/Antigens, CD20; 0/Antigens, CD30; 0/EBV-associated membrane antigen, Epstein-Barr virus; 0/Viral Matrix Proteins

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