Document Detail


Diagnostic accuracy of blood lactate-to-pyruvate molar ratio in the differential diagnosis of congenital lactic acidosis.
MedLine Citation:
PMID:  17384007     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Although the blood lactate-to-pyruvate (L:P) molar ratio is used to distinguish between pyruvate dehydrogenase deficiency (PDH-D) and other causes of congenital lactic acidosis (CLA), its diagnostic accuracy for differentiating between these 2 types of CLA has not been evaluated formally. METHODS: We conducted a retrospective study of all patients followed for mitochondrial diseases between 1985 and 2005 in a tertiary care pediatric hospital. RESULTS: At the recommended cut point of approximately 25, individual median L:P ratio demonstrated low sensitivity and specificity (77% and 91%, respectively) for differentiating between patients with enzymatically proven PDH-D (n = 11) and those with mitochondrial disease but normal pyruvate dehydrogenase (PDH) activity (non-PDH; n = 35). We observed a strong positive association between L:P ratio and blood lactate in non-PDH CLA, whereas this association was weak in PDH-D CLA. Consequently, patient classification based on median L:P ratio showed improved diagnostic accuracy at higher lactate concentrations: for lactate <2.5 mmol/L the area under the ROC curve was not statistically different from 0.5 (P = 0.3), whereas it was statistically different for lactate >2.5 mmol/L. In the 2.5 to 5.0 mmol/L lactate category, the sensitivity and specificity at an optimal cut point of 18.4 were 93% (95% CI, 77%-99%) and 71% (95% CI, 20%-96%), respectively; for lactate >5.0 mmol/L, with an optimal cut point of 25.8, sensitivity and specificity were 96% (95% CI, 77%-99%) and 100% (95% CI, 59%-100%), respectively. CONCLUSION: Usefulness of the L:P ratio for differentiating non-PDH and PDH-D types of CLA increases at higher lactate concentrations.
Authors:
François-Guillaume Debray; Grant A Mitchell; Pierre Allard; Brian H Robinson; James A Hanley; Marie Lambert
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Publication Detail:
Type:  Journal Article     Date:  2007-03-23
Journal Detail:
Title:  Clinical chemistry     Volume:  53     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-30     Completed Date:  2007-06-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  916-21     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, Ste-Justine Hospital, Université de Montréal, Montreal, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Acidosis, Lactic / congenital,  diagnosis*
Child
Diagnosis, Differential
Female
Hospitals, Pediatric
Humans
Lactic Acid / blood*
Male
Mitochondrial Diseases / diagnosis
Pyruvate Dehydrogenase Complex Deficiency Disease / diagnosis
Pyruvic Acid / blood*
ROC Curve
Retrospective Studies
Sensitivity and Specificity
Chemical
Reg. No./Substance:
127-17-3/Pyruvic Acid; 50-21-5/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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