Document Detail


Diagnostic utility of major basic protein, eotaxin-3, and leukotriene enzyme staining in eosinophilic esophagitis.
MedLine Citation:
PMID:  22777338     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Features of eosinophilic esophagitis (EoE) and gastroesophageal reflux disease (GERD) overlap. We aimed to determine whether staining for tissue biomarkers would differentiate EoE from GERD, suggesting utility for diagnosis of EoE.
METHODS: In this case-control study, EoE patients defined by consensus guidelines were compared to GERD patients with eosinophils on esophageal biopsy. Immunohistochemistry was performed for major basic protein (MBP), eotaxin-3, leukotriene A4 hydrolase (LTA4H), and leukotriene C4 synthase (LTC4S). After masking, the maximum staining density (cells per mm(2)) was quantified for each marker and compared between groups. Receiver operator characteristic curves were constructed, and the area under the curve (AUC) calculated to assess the diagnostic utility of each of the biomarkers alone and in combination with eosinophil counts.
RESULTS: There were 51 EoE cases (mean age 24; mean 143 eosinophils per high-power field (eos per h.p.f.)) and 54 GERD controls (mean age 34; mean 20 eos per h.p.f.). The MBP density was higher in EoE than in GERD (1479 vs. 59 cells per mm(2); P<0.001), as was the eotaxin-3 density (2219 vs. 479; P<0.001). There were no differences for LTA4H and LTC4S. MBP density and eosinophil count correlated (R=0.81; P<0.001); correlation with eotaxin-3 was weaker (R=0.25; P=0.01). The AUC for diagnosis of EoE was 0.96 for MBP, 0.87 for eotaxin-3, 0.58 for LTA4H, 0.66 for LTC4S, and 0.99 for the combination of MBP, eotaxin-3, and eosinophil count.
CONCLUSIONS: Patients with EoE had substantially higher levels of MBP and eotaxin-3 staining than GERD patients. These markers may have utility as a diagnostic assay for EoE.
Authors:
Evan S Dellon; Xiaoxin Chen; C Ryan Miller; John T Woosley; Nicholas J Shaheen
Related Documents :
22314448 - Seasonal variation in types of stroke and its common risk factors.
22899598 - Anatomic correlates of supra-normal ankle brachial indices.
22622408 - Phenotypic differences in dyt1 between ethnic groups.
21996048 - Use of mean spot urine sodium concentrations to estimate daily sodium intake in patient...
24489238 - A3.21 microrna-34a and microrna-155 in systemic sclerosis: possible epigenetic biomarke...
2298368 - T-cell activation in crohn's disease. increased levels of soluble interleukin-2 recepto...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-07-10
Journal Detail:
Title:  The American journal of gastroenterology     Volume:  107     ISSN:  1572-0241     ISO Abbreviation:  Am. J. Gastroenterol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-04     Completed Date:  2012-12-07     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  0421030     Medline TA:  Am J Gastroenterol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1503-11     Citation Subset:  IM    
Affiliation:
Center for Esophageal Diseases and Swallowing, Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA. edellon@med.unc.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Area Under Curve
Biological Markers / metabolism
Case-Control Studies
Chemokines, CC / metabolism*
Diagnosis, Differential
Eosinophil Major Basic Protein / metabolism*
Eosinophilic Esophagitis / diagnosis*,  metabolism*
Eosinophils* / metabolism
Female
Gastroesophageal Reflux / diagnosis,  metabolism
Humans
Immunohistochemistry
Immunologic Factors / metabolism
Leukocyte Count
Leukotriene A4 / metabolism
Leukotriene C4 / metabolism
Leukotrienes / metabolism*
Male
Predictive Value of Tests
ROC Curve
Grant Support
ID/Acronym/Agency:
K23 DK090073/DK/NIDDK NIH HHS; K23DK090073/DK/NIDDK NIH HHS; KL2 RR025746/RR/NCRR NIH HHS; KL2RR025746/RR/NCRR NIH HHS; P30 DK034987/DK/NIDDK NIH HHS; P30DK034987/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/CCL26 protein, human; 0/Chemokines, CC; 0/Immunologic Factors; 0/Leukotriene A4; 0/Leukotrienes; 72025-60-6/Leukotriene C4; EC 3.1.27.-/Eosinophil Major Basic Protein
Comments/Corrections
Comment In:
Am J Gastroenterol. 2013 Mar;108(3):453-4   [PMID:  23459054 ]
Am J Gastroenterol. 2013 Mar;108(3):452-3   [PMID:  23459053 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The Performance of Process Measures in Hepatitis C.
Next Document:  Concomitant symptoms itemized in the Reflux Disease Questionnaire are associated with attenuated hea...