Document Detail


Diagnostic ELISA for parietal cell autoantibody using tomato lectin-purified gastric H+/K(+)-ATPase (proton pump).
MedLine Citation:
PMID:  1319758     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Circulating parietal cell autoantibodies, a useful diagnostic marker for autoimmune gastritis and pernicious anaemia, are currently routinely tested by serum immunofluorescence reactivity with frozen sections of rodent stomach. The major molecular targets of these parietal cell autoantibodies have recently been demonstrated to be the alpha- and the beta-subunits of the gastric H+/K(+)-ATPase (proton pump). We have demonstrated that tomato lectin binds specifically to the beta-subunit of the proton pump and concomittantly co-purifies the alpha-subunit. In the present study, we have exploited the latter observation for the development of a diagnostic ELISA for the detection of parietal cell autoantibodies and compared the performance of this assay with an ELISA using crude gastric membranes. The ELISAs were tested on 72 parietal cell autoantibody-positive sera, 72 parietal cell autoantibody-negative sera and 72 disease-control sera. The ELISA using lectin-purified canine proton pump was superior to that using crude canine gastric membranes in that it was about two-fold more sensitive (82% vs. 43%). With an assay sensitivity of 82% and a specificity of 90%, we propose that the ELISA using the lectin-purified proton pump is a rapid, simple, sensitive and specific diagnostic immunoassay for parietal cell autoantibodies.
Authors:
J S Chuang; J M Callaghan; P A Gleeson; B H Toh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Autoimmunity     Volume:  12     ISSN:  0891-6934     ISO Abbreviation:  Autoimmunity     Publication Date:  1992  
Date Detail:
Created Date:  1992-08-04     Completed Date:  1992-08-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8900070     Medline TA:  Autoimmunity     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  1-7     Citation Subset:  IM    
Affiliation:
Department of Pathology, Monash University Medical School, Monash University, Prahan, Victoria, Australia.
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MeSH Terms
Descriptor/Qualifier:
Autoantibodies / analysis*
Enzyme-Linked Immunosorbent Assay
Gastric Mucosa / enzymology
Humans
Lectins
Parietal Cells, Gastric / enzymology,  immunology*
Plant Lectins*
Sodium-Potassium-Exchanging ATPase / immunology*
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/Lectins; 0/Plant Lectins; 0/tomato lectin; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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