Document Detail


Diagnosis of visceral leishmaniasis by the polymerase chain reaction using blood, bone marrow and lymph node samples from patients from the Sudan.
MedLine Citation:
PMID:  9217699     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have evaluated the sensitivity of the polymerase chain reaction (PCR) as a diagnostic tool for Leishmania donovani using blood, bone marrow and lymph node samples from Sudanese patients with a confirmed infection. Forty patients were diagnosed by microscopic examination of bone marrow or lymph node samples. The PCR was able to detect parasite DNA in 37 out of 40 blood samples. In bone marrow and lymph node samples, the PCR was able to detect parasite DNA in all 7 and 6 samples, respectively. We suggest that the PCR should be considered as a valuable and sensitive tool for the diagnosis of L. donovani infection. However, if PCR diagnosis is to supplement or even replace microscopic diagnosis in developing countries, a large number of patients with no apparent signs of infection and patients with other diseases have to be tested in order to evaluate its true potential.
Authors:
K Andresen; S Gasim; A M Elhassan; E A Khalil; D C Barker; T G Theander; A Kharazmi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tropical medicine & international health : TM & IH     Volume:  2     ISSN:  1360-2276     ISO Abbreviation:  Trop. Med. Int. Health     Publication Date:  1997 May 
Date Detail:
Created Date:  1997-08-07     Completed Date:  1997-08-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9610576     Medline TA:  Trop Med Int Health     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  440-4     Citation Subset:  IM    
Affiliation:
Department of Clinical Microbiology, RHIMA University Hospital (Rigshospitalet), Copenhagen, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow / parasitology
DNA, Protozoan / isolation & purification*
Humans
Leishmania donovani / genetics*
Leishmaniasis, Visceral / blood,  diagnosis*
Lymph Nodes / parasitology
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Sudan
Chemical
Reg. No./Substance:
0/DNA, Protozoan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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