Document Detail

Diagnosis of respiratory syncytial virus infection: comparison of reverse transcription-PCR to viral culture and serology in adults with respiratory illness.
MedLine Citation:
PMID:  11880399     Owner:  NLM     Status:  MEDLINE    
Diagnosis of respiratory syncytial virus (RSV) during acute infection in adults is difficult because of the poor sensitivity of viral culture and antigen detection. A recently developed single-tube nested reverse transcription-PCR (RT-PCR) was compared to viral culture and serology by enzyme immunoassay for the diagnosis of RSV in adults with respiratory illness. Nasal swab samples were collected during respiratory illnesses from five groups of subjects: healthy young adults, healthy elderly adults, adults with chronic heart and lung disease, nursing home residents, and adults admitted to the hospital during the winter with cardiopulmonary illnesses. Of 1,112 samples for which all three tests were available, 117 were positive by at least one method and 995 were negative by all methods. One hundred ten were considered true positives because culture and/or serology was positive. Of these, 80 (73%) were PCR positive compared to 43 (39%) that were culture positive. Seven PCR results were considered false positives due to negative culture and serology. The overall RT-PCR sensitivity was 73%, and specificity was 99%. These data indicate that RT-PCR is an excellent method for the diagnosis of acute RSV infection in adults.
Ann R Falsey; Maria A Formica; Edward E Walsh
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  40     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-03-06     Completed Date:  2002-04-18     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  817-20     Citation Subset:  IM    
Department of Medicine, Rochester General Hospital, University of Rochester School of Medicine and Dentistry, Rochester, New York 14621, USA.
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MeSH Terms
Middle Aged
Respiratory Syncytial Virus Infections / diagnosis*
Respiratory Syncytial Virus, Human / isolation & purification*
Reverse Transcriptase Polymerase Chain Reaction*
Sensitivity and Specificity
Grant Support
R01-45969//PHS HHS

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