Document Detail

Diagnosis of pulmonary histiocytosis X by immunodetection of Langerhans cells in bronchoalveolar lavage fluid.
MedLine Citation:
PMID:  6372496     Owner:  NLM     Status:  MEDLINE    
Based on the finding that Langerhans cells and histiocytosis X cells react with the monoclonal antibody OKT6, raised against a subset of thymocytes, we used this antibody to study the cells collected by bronchoalveolar lavage (BAL) from 131 patients, including 18 with pulmonary histiocytosis X, 43 with pulmonary sarcoidosis, 67 with miscellaneous pulmonary disorders, and 3 controls. Immunofluorescence studies demonstrated the presence of OKT6-reactive cells in all patients with pulmonary histiocytosis X (mean +/- SEM, 5.29% +/- 1.14% of all cells in BAL fluid). Immunoelectron microscopic studies revealed that the cells labeled in these patients (n = 13) contained Langerhans granules. The number of fluorescent cells in the other 113 patients was significantly smaller (mean +/- SEM, 0.20% +/- 0.04% of all cells; P less than 0.001). In the 3 control patients, in the 43 patients with sarcoidosis, and in 61 of the 67 patients with miscellaneous disorders unrelated to histiocytosis X, no cells or less than 1% of the total were labeled; however, in the 6 remaining patients in this miscellaneous group, 1.3 to 2.8% of all cells in BAL were labeled. In 3 of these 6 patients, immunoelectronmicroscopic examination showed that the cells labeled by OKT6 had the general characteristics of Langerhans cells but lacked Langerhans granules. OKT3, OKT4, and OKT8 monoclonal antibodies did not stain histiocytosis X cells in BAL fluid.
S Chollet; P Soler; P Dournovo; M S Richard; V J Ferrans; F Basset
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of pathology     Volume:  115     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  1984 May 
Date Detail:
Created Date:  1984-05-30     Completed Date:  1984-05-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  225-32     Citation Subset:  AIM; IM    
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MeSH Terms
Antibodies, Monoclonal / diagnostic use*
Cell Count
Cell Membrane / immunology,  ultrastructure
Fluorescent Antibody Technique
Histiocytosis, Langerhans-Cell / pathology*
Langerhans Cells* / immunology,  ultrastructure
Lung Diseases / pathology*
Lymphocytes / immunology
Microscopy, Electron
Pulmonary Alveoli / pathology*
Sarcoidosis / pathology
Reg. No./Substance:
0/Antibodies, Monoclonal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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