| Diagnosis of progressive multifocal leukoencephalopathy by stereotactic brain biopsy utilizing immunohistochemistry and the polymerase chain reaction. | |
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MedLine Citation:
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PMID: 7847007 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This report describes the diagnosis of progressive multifocal leukoencephalopathy (PML) in nine patients using cytopathologic and histopathologic examination of computed tomographically guided stereotactic brain biopsies in combination with immunostaining for SV-40-related antigen and the polymerase chain reaction (PCR) for the JC virus. In four patients the diagnosis of PML was based on the microscopic appearance of the biopsies and immunostaining for SV-40-related antigen. In one of these patients the diagnosis was also supported by PCR for the JC virus. In two patients whose biopsies were only suggestive of PML, a definitive diagnosis was possible utilizing immunohistochemistry and PCR. In another case the histopathologic features were atypical of PML, and the diagnosis was established with immunostaining and PCR. The diagnosis of PML was established by PCR alone in two patients whose biopsies showed only suggestive or nonspecific findings. We conclude that the accuracy of stereotactic biopsy in the diagnosis of PML is enhanced by using a combination of light microscopy, immunohistochemistry and PCR. |
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Authors:
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S A Silver; R R Arthur; Y S Erozan; M E Sherman; J C McArthur; S Uematsu |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Acta cytologica Volume: 39 ISSN: 0001-5547 ISO Abbreviation: Acta Cytol. Publication Date: 1995 Jan-Feb |
Date Detail:
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Created Date: 1995-03-06 Completed Date: 1995-03-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370307 Medline TA: Acta Cytol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 35-44 Citation Subset: IM; X |
Affiliation:
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Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antigens, Polyomavirus Transforming / analysis Base Sequence Biopsy / methods Brain / pathology*, radiography DNA, Viral / analysis, genetics Genome, Viral HIV Infections / complications Humans Immunohistochemistry JC Virus / genetics Leukoencephalopathy, Progressive Multifocal / complications, diagnosis*, pathology* Magnetic Resonance Imaging Male Middle Aged Molecular Sequence Data Oligonucleotide Probes / analysis, chemistry, genetics Polymerase Chain Reaction Tomography, X-Ray Computed |
| Grant Support | |
ID/Acronym/Agency:
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AI-72634/AI/NIAID NIH HHS; NS-26643/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Polyomavirus Transforming; 0/DNA, Viral; 0/Oligonucleotide Probes |
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