| Diagnosis of leptomeningeal disease in diffuse large B-cell lymphomas of the central nervous system by flow cytometry and cytopathology. | |
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MedLine Citation:
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PMID: 20727005 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Reliable detection of leptomeningeal disease has the potential of facilitating the diagnosis of central nervous system (CNS) lymphoma and is important for therapeutic considerations. Currently, the standard diagnostic procedure for the detection of lymphoma in the cerebrospinal fluid is cytopathology. To improve the limited specificity and sensitivity of cytopathology, flow cytometry has been suggested as an alternative. Here, we evaluated multi-parameter flow cytometry in combination with conventional cytopathology in cerebrospinal fluid (CSF) samples from 30 patients with primary CNS lymphoma and seven patients with secondary CNS lymphoma. Overall, in 11 of 37 (29.7%) patients with CNS lymphoma, lymphoma cells were detected in CSF by flow cytometry, while cytopathology was less sensitive displaying unequivocally malignant CSF cells in only seven of all 37 (18.9%) patients. Six (16.2%) patients showed cytopathological results suspicious of lymphoma; however, in only one of these patients, the diagnosis of CSF lymphoma cells could be confirmed by flow cytometry. In primary CNS lymphomas (PCNSL), seven of 30 (23.3%) patients were positive for CSF lymphoma cells in flow cytometry, in contrast to four (13.3%) patients with PCNSL with definitely positive cytopathology. In summary, our results suggest that multi-parameter flow cytometry increases the sensitivity and specificity of leptomeningeal disease detection in CNS lymphomas. Both methods should be applied concurrently for complementary diagnostic assessment in patients with CNS lymphoma. |
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Authors:
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Roland Schroers; Alexander Baraniskin; Christoph Heute; Matthias Vorgerd; Anna Brunn; Jan Kuhnhenn; Annika Kowoll; Andriy Alekseyev; Wolff Schmiegel; Uwe Schlegel; Martina Deckert; Hendrik Pels |
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Publication Detail:
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Type: Clinical Trial; Journal Article Date: 2010-10-28 |
Journal Detail:
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Title: European journal of haematology Volume: 85 ISSN: 1600-0609 ISO Abbreviation: Eur. J. Haematol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-12 Completed Date: 2010-12-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8703985 Medline TA: Eur J Haematol Country: England |
Other Details:
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Languages: eng Pagination: 520-8 Citation Subset: IM |
Copyright Information:
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© 2010 John Wiley & Sons A/S. |
Affiliation:
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Department of Medicine, Hematology and Oncology, Ruhr-University of Bochum, Bochum, Germany. Roland.Schroers@rub.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Diagnosis, Differential Female Flow Cytometry* Humans Lymphoma, Large B-Cell, Diffuse / cerebrospinal fluid*, pathology* Male Meningeal Neoplasms / cerebrospinal fluid*, pathology*, secondary* Middle Aged |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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