| Diagnosis of human immunodeficiency virus infection by enzyme-linked immunospot assays in a prospectively followed cohort of infants of human immunodeficiency virus-seropositive women. | |
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MedLine Citation:
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PMID: 1523074 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The enzyme-linked immunospot (ELISPOT), a method for quantifying specific and total antibody-secreting cells, was used for the diagnosis of human immunodeficiency virus (HIV) infection in a prospectively followed cohort of infants born to HIV-infected women. From July 1, 1987, to June 1, 1990, 127 infants with known HIV infection status were studied. Seventeen of 22 HIV-infected infants had specific HIV-specific antibody-secreting cells (ASC). Among the infected infants rates of ASC positivity increased during the first year of life, from 25% in the first 5 days of life to 78% after 6 months. Two of the five ASC-negative infected infants were further characterized as hypo- or dysgammaglobulinemic by an adjunct ELISPOT assay for total immunoglobulin-secreting cells. Excluding hypo- or dysgammaglobulinemic infants from the analysis, the rate of ASC positivity among infected infants was 85% (17 of 20) after the age of 6 months. None of the 95 uninfected infants had a positive ELISPOT assay, including 55 who were tested in the first 3 months of life. Thus in this series the specificity was 100%. ELISPOT methodology can be a useful technique for the diagnosis of HIV infection in infants of HIV-seropositive mothers. |
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Authors:
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S Nesheim; F Lee; M Sawyer; D Jones; M Lindsay; B Slade; N Shaffer; R Holmes; R Ashby; V Grimes |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Pediatric infectious disease journal Volume: 11 ISSN: 0891-3668 ISO Abbreviation: Pediatr. Infect. Dis. J. Publication Date: 1992 Aug |
Date Detail:
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Created Date: 1992-10-09 Completed Date: 1992-10-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8701858 Medline TA: Pediatr Infect Dis J Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 635-9 Citation Subset: IM; X |
Affiliation:
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Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30303. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Female HIV Infections / diagnosis* Humans Immunoenzyme Techniques* Infant Infant, Newborn Predictive Value of Tests Pregnancy Prenatal Exposure Delayed Effects* Prospective Studies |
| Grant Support | |
ID/Acronym/Agency:
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646/CCU404456-02//PHS HHS; R01-HD-7-2925/HD/NICHD NIH HHS; R03-AI30963/AI/NIAID NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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