Document Detail


Diagnosis of human immunodeficiency virus infection by enzyme-linked immunospot assays in a prospectively followed cohort of infants of human immunodeficiency virus-seropositive women.
MedLine Citation:
PMID:  1523074     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The enzyme-linked immunospot (ELISPOT), a method for quantifying specific and total antibody-secreting cells, was used for the diagnosis of human immunodeficiency virus (HIV) infection in a prospectively followed cohort of infants born to HIV-infected women. From July 1, 1987, to June 1, 1990, 127 infants with known HIV infection status were studied. Seventeen of 22 HIV-infected infants had specific HIV-specific antibody-secreting cells (ASC). Among the infected infants rates of ASC positivity increased during the first year of life, from 25% in the first 5 days of life to 78% after 6 months. Two of the five ASC-negative infected infants were further characterized as hypo- or dysgammaglobulinemic by an adjunct ELISPOT assay for total immunoglobulin-secreting cells. Excluding hypo- or dysgammaglobulinemic infants from the analysis, the rate of ASC positivity among infected infants was 85% (17 of 20) after the age of 6 months. None of the 95 uninfected infants had a positive ELISPOT assay, including 55 who were tested in the first 3 months of life. Thus in this series the specificity was 100%. ELISPOT methodology can be a useful technique for the diagnosis of HIV infection in infants of HIV-seropositive mothers.
Authors:
S Nesheim; F Lee; M Sawyer; D Jones; M Lindsay; B Slade; N Shaffer; R Holmes; R Ashby; V Grimes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Pediatric infectious disease journal     Volume:  11     ISSN:  0891-3668     ISO Abbreviation:  Pediatr. Infect. Dis. J.     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-10-09     Completed Date:  1992-10-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8701858     Medline TA:  Pediatr Infect Dis J     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  635-9     Citation Subset:  IM; X    
Affiliation:
Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30303.
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MeSH Terms
Descriptor/Qualifier:
Female
HIV Infections / diagnosis*
Humans
Immunoenzyme Techniques*
Infant
Infant, Newborn
Predictive Value of Tests
Pregnancy
Prenatal Exposure Delayed Effects*
Prospective Studies
Grant Support
ID/Acronym/Agency:
646/CCU404456-02//PHS HHS; R01-HD-7-2925/HD/NICHD NIH HHS; R03-AI30963/AI/NIAID NIH HHS

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