Document Detail


Diagnosis of human congenital cytomegalovirus infection by amplification of viral DNA from dried blood spots on perinatal cards.
MedLine Citation:
PMID:  16645211     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Congenital human cytomegalovirus (HCMV) infection affects 1% of children and is the most common infectious cause of sensorineural hearing loss. Due to the difficulty of diagnosing deafness and other neurological disorders in infants, affected individuals may not be recognized until much later when active infection has resolved and culture is no longer informative. To overcome this problem, congenital HCMV infection was diagnosed retrospectively by testing residual blood samples collected from newborns and dried on perinatal cards as part of the North Carolina Newborn Screening Program. We modified the Qiagen method for purifying DNA from dried blood spots to increase the sample size and recovery of the lysate. A multiplex, real-time TaqMan polymerase chain reaction assay on an ABI 7900 instrument measured a highly conserved segment of the HCMV polymerase gene and the APOB human control gene. HCMV DNA was detected in blood dried on perinatal cards from all seven infants with culture-proven congenital infection, and all 24 negative control cases lacked detectable HCMV DNA. Our findings suggest that it is possible to diagnose congenital HCMV infection using dried blood collected up to 20 months earlier. Further studies are warranted on patients with hearing loss or other neurological deficits to determine the percentage that is attributable to congenital HCMV infection.
Authors:
Lori Scanga; Shu Chaing; Cynthia Powell; Arthur S Aylsworth; Lizzie J Harrell; Nancy G Henshaw; Chris J Civalier; Leigh B Thorne; Karen Weck; Jessica Booker; Margaret L Gulley
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of molecular diagnostics : JMD     Volume:  8     ISSN:  1525-1578     ISO Abbreviation:  J Mol Diagn     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-04-28     Completed Date:  2006-08-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  100893612     Medline TA:  J Mol Diagn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  240-5     Citation Subset:  IM    
Affiliation:
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, 27599-7525, USA.
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MeSH Terms
Descriptor/Qualifier:
Cytomegalovirus / genetics*,  isolation & purification*
Cytomegalovirus Infections / blood,  congenital*,  diagnosis*,  virology
DNA, Viral / blood*,  genetics*
Diagnostic Tests, Routine / instrumentation*,  methods
Humans
Infant, Newborn
Polymerase Chain Reaction
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/DNA, Viral
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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