| Diagnosis of human brucellosis with ELISA. | |
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MedLine Citation:
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PMID: 6123753 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ELISA tests for total (IgG + IgM + IgA), IgG, and IgM anti-Brucella antibodies, which utilised only commercially available reagents, were used to diagnose human brucellosis. Assays for total antibodies in sera from 22 patients with confirmed acute brucellosis, 1 patient with probable acute brucellosis, and 3 patients with probable chronic brucellosis gave readings that were more than double those found in hundreds of control sera. All sera from patients with acute and chronic brucellosis had significantly elevated IgG levels. Although there were a few acute patients with IgM levels only slightly higher than those of some controls, most patients with acute disease could readily be differentiated from both the non-brucellosis patients and patients with chronic brucellosis by measuring macroglobulins. Both the IgG and IgM levels of sera from acute patients persisted for at least 8 months. The results of this show that ELISA is an excellent method for screening large populations for Brucella antibodies and for differentiation between the acute and chronic phases of the disease. |
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Authors:
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J E Sippel; N A El-Masry; Z Farid |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Lancet Volume: 2 ISSN: 0140-6736 ISO Abbreviation: Lancet Publication Date: 1982 Jul |
Date Detail:
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Created Date: 1982-08-14 Completed Date: 1982-08-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985213R Medline TA: Lancet Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 19-21 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acute Disease Antibodies, Bacterial / analysis Brucella / immunology Brucellosis / diagnosis* Enzyme-Linked Immunosorbent Assay* / methods Humans Immunoenzyme Techniques* Immunoglobulin G / analysis Immunoglobulin M / analysis |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 0/Immunoglobulin G; 0/Immunoglobulin M |
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