Document Detail

Diagnosis of human brucellosis with ELISA.
MedLine Citation:
PMID:  6123753     Owner:  NLM     Status:  MEDLINE    
ELISA tests for total (IgG + IgM + IgA), IgG, and IgM anti-Brucella antibodies, which utilised only commercially available reagents, were used to diagnose human brucellosis. Assays for total antibodies in sera from 22 patients with confirmed acute brucellosis, 1 patient with probable acute brucellosis, and 3 patients with probable chronic brucellosis gave readings that were more than double those found in hundreds of control sera. All sera from patients with acute and chronic brucellosis had significantly elevated IgG levels. Although there were a few acute patients with IgM levels only slightly higher than those of some controls, most patients with acute disease could readily be differentiated from both the non-brucellosis patients and patients with chronic brucellosis by measuring macroglobulins. Both the IgG and IgM levels of sera from acute patients persisted for at least 8 months. The results of this show that ELISA is an excellent method for screening large populations for Brucella antibodies and for differentiation between the acute and chronic phases of the disease.
J E Sippel; N A El-Masry; Z Farid
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Lancet     Volume:  2     ISSN:  0140-6736     ISO Abbreviation:  Lancet     Publication Date:  1982 Jul 
Date Detail:
Created Date:  1982-08-14     Completed Date:  1982-08-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985213R     Medline TA:  Lancet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  19-21     Citation Subset:  AIM; IM    
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MeSH Terms
Acute Disease
Antibodies, Bacterial / analysis
Brucella / immunology
Brucellosis / diagnosis*
Enzyme-Linked Immunosorbent Assay* / methods
Immunoenzyme Techniques*
Immunoglobulin G / analysis
Immunoglobulin M / analysis
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Immunoglobulin G; 0/Immunoglobulin M

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