Document Detail

Diagnosis of histoplasmosis by detection of the internal transcribed spacer region of fungal rRNA gene from a paraffin-embedded skin sample from a dog in Japan.
MedLine Citation:
PMID:  12814889     Owner:  NLM     Status:  MEDLINE    
The lesions of histoplasmosis in dogs in Japan differ from those in dogs in North America. Affected dogs in Japan have had multiple granulomatous or ulcerated foci in skin or gingiva and have not had pulmonary or gastrointestinal lesions. The present report introduces a polymerase chain reaction (PCR) diagnosis of canine histoplasmosis and the characteristic of disease in Japan. The surgically removed skin ulcerate samples from a 5-years-old female Shiba-inu native to Japan without traveling out of the country were evaluated. Tissue samples had many yeast-like organisms in the macrophages. DNA was extracted from paraffin-embedded tissue samples. A nested PCR technique was applied. The detected sequence of the internal transcribed spacer of ribosomal RNA gene had 99.7% in homology with Ajellomyces capsulatus (the teleomorph of Histoplasma capsulatum). Clinical manifestations, historical background of equine epizootic lymphangitis in Japan, and a human autochthonous case of histoplasmosis farciminosi indicated that this dog might have been infected with H. capsulatum var. farciminosum as a heteroecism.
Yachiyo Ueda; Ayako Sano; Miki Tamura; Tomo Inomata; Katsuhiko Kamei; Koji Yokoyama; Fukuko Kishi; Junko Ito; Yuzuru Mikami; Makoto Miyaji; Kazuko Nishimura
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Veterinary microbiology     Volume:  94     ISSN:  0378-1135     ISO Abbreviation:  Vet. Microbiol.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-19     Completed Date:  2003-10-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705469     Medline TA:  Vet Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  219-24     Citation Subset:  IM    
Ueda Animal Clinic, 3-16-2, Nakamura-Minami, Nerima-Ku, Tokyo 176-0025, Japan.
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MeSH Terms
Base Sequence
DNA, Ribosomal Spacer / analysis*,  chemistry,  genetics
Dog Diseases / diagnosis*,  microbiology
Histoplasma / genetics,  isolation & purification*
Histoplasmosis / diagnosis,  microbiology,  veterinary*
Macrophages / microbiology
Molecular Sequence Data
Polymerase Chain Reaction / methods,  veterinary
RNA, Fungal / genetics*,  isolation & purification
RNA, Ribosomal, 5.8S / genetics
Sequence Homology, Nucleic Acid
Skin / microbiology,  pathology
Reg. No./Substance:
0/DNA, Ribosomal Spacer; 0/RNA, Fungal; 0/RNA, Ribosomal, 5.8S

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