Document Detail


Diagnosis of cytomegalovirus infections by qualitative and quantitative PCR in HIV infected patients.
MedLine Citation:
PMID:  12163904     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A high incidence of cytomegalovirus (CMV) infections is observed in Brazil. These viruses are causatives of significant morbidity and mortality among patients with advanced human immunodeficiency virus (HIV) infection. This work, shows the application of a PCR on determination of CMV load in the buffy coat and plasma. We analyzed the samples of 247 HIV infected patients in order to diagnose CMV infection and disease. We developed a semi-quantitative PCR that amplifies part of the glycoprotein B (gB) gene of CMV. The semi-quantitative PCR was carried out only in positive clinical samples in a qualitative PCR confirmed by a nested-PCR. CD4 lymphocyte count, HIV viral load and CMV disease symptom were correlated with CMV load. CMV genome was detected in the buffy coat of 82 of 237 (34.6%) patients, in 10 of these the CMV load was determined varying between 928 and 332 880 viral copies/microg DNA. None of these 237 patients developed any suggestive manifestation of CMV disease. For the other 10 HIV infected patients selected based on the suspicion of CMV disease, CMV genome was detected in only one case. This patient presented a high CMV load, 8 000 000 copies/microg DNA, and developed a disseminated form of CMV disease including hepatitis and retinitis. Our results were greatly influenced by the impact of the highly active antiretroviral therapy that reduced incidence of CMV viremia and occurrence of CMV disease in the HIV infected patients.
Authors:
Aldo de Albuquerque Cunha; Lauro Juliano Marin; Victor Hugo Aquino; Luiz Tadeu Moraes Figueiredo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Revista do Instituto de Medicina Tropical de São Paulo     Volume:  44     ISSN:  0036-4665     ISO Abbreviation:  Rev. Inst. Med. Trop. Sao Paulo     Publication Date:    2002 May-Jun
Date Detail:
Created Date:  2002-08-06     Completed Date:  2002-10-18     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7507484     Medline TA:  Rev Inst Med Trop Sao Paulo     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  127-32     Citation Subset:  IM    
Affiliation:
Unidade Multidepartamental de Pesquisa em Virologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
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MeSH Terms
Descriptor/Qualifier:
Adult
Brazil / epidemiology
CD4 Lymphocyte Count
Cytomegalovirus / isolation & purification
Cytomegalovirus Infections / diagnosis*,  epidemiology,  virology
DNA, Viral / analysis
Female
HIV Infections / epidemiology,  virology*
Humans
Male
Middle Aged
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Viral Load
Chemical
Reg. No./Substance:
0/DNA, Viral

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