Document Detail


Diagnosis of anatoxin-a poisoning in dogs from North America.
MedLine Citation:
PMID:  18182518     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Anatoxin-a, a toxin produced by several genera of blue-green algae, is considered a potent neurotoxin. Ingestion of water contaminated with the toxin results in acute neurological signs and often death. This report describes fatal cases of anatoxin-a ingestion in 6 dogs, with confirmation of anatoxin-a exposure by liquid chromatography/tandem mass spectrometry (LC-MS/MS/MS). In 1 outbreak, 3 dogs developed seizures and died within an hour after swimming in a river in California, while the other outbreak involved 3 dogs that died within 1 hour after swimming in a pond in Ontario. Anatoxin-a poisoning is rarely reported in dogs as a cause of acute neurological signs and death. However, increased occurrences of blue-green algae blooms in North America make this neurotoxin an important consideration in the diagnosis of sudden death associated with environmental water exposure. This brief communication reports on the isolation and detection of anatoxin-a from environmental water sources and the stomach contents of North American dogs dying of acute neurotoxicosis. This demonstrates the first documented cases of anatoxin-a poisoning in dogs in North America and the importance of LC-MS/MS/MS in identifying neurotoxins responsible for sudden death in cases of suspected blue-green algae toxicosis; especially those cases showing no gross or histological lesions.
Authors:
Birgit Puschner; Brent Hoff; Elizabeth R Tor
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc     Volume:  20     ISSN:  1040-6387     ISO Abbreviation:  J. Vet. Diagn. Invest.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-09     Completed Date:  2008-10-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9011490     Medline TA:  J Vet Diagn Invest     Country:  United States    
Other Details:
Languages:  eng     Pagination:  89-92     Citation Subset:  IM    
Affiliation:
California Animal Health and Food Safety Laboratory System, Toxicology Laboratory, University of California, West Health Sciences Drive, Davis, CA 95616, USA. bpuschner@ucdavis.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, Liquid
Cyanobacteria*
Dog Diseases / chemically induced*
Dogs
Gas Chromatography-Mass Spectrometry / veterinary
Gastrointestinal Contents / chemistry
Liver / chemistry
Neurotoxins / isolation & purification,  metabolism,  poisoning*
Seizures / chemically induced,  veterinary*
Tandem Mass Spectrometry
Tropanes / isolation & purification,  metabolism,  poisoning*
Water
Chemical
Reg. No./Substance:
0/Neurotoxins; 0/Tropanes; 0/anatoxin a; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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