Document Detail


Diagnosis of amyloidosis and differentiation from chronic, idiopathic enterocolitis in rhesus (Macaca mulatta) and pig-tailed (M. nemestrina) macaques.
MedLine Citation:
PMID:  23759529     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Amyloidosis is a progressive and ultimately fatal disease in which amyloid, an insoluble fibrillar protein, is deposited inappropriately in multiple organs, eventually leading to organ dysfunction. Although this condition commonly affects macaques, there is currently no reliable method of early diagnosis. Changes in clinical pathology parameters have been associated with amyloidosis but occur in late stages of disease, are nonspecific, and resemble those seen in chronic, idiopathic enterocolitis. A review of animal records revealed that amyloidosis was almost always diagnosed postmortem, with prevalences of 15% and 25% in our rhesus and pig-tailed macaque colonies, respectively. As a noninvasive, high-throughput diagnostic approach to improve antemortem diagnosis of amyloidosis in macaques, we evaluated serum amyloid A (SAA), an acute-phase protein and the precursor to amyloid. Using necropsy records and ELISA analysis of banked serum, we found that SAA is significantly elevated in both rhesus and pig-tailed macaques with amyloid compared with those with chronic enterocolitis and healthy controls. At necropsy, 92% of rhesus and 83% of pig-tailed had amyloid deposition in either the intestines or liver. Minimally invasive biopsy techniques including endoscopy of the small intestine, mucosal biopsy of the colon, and ultrasound-guided trucut biopsy of the liver were used to differentiate macaques in our colonies with similar clinical presentations as either having amyloidosis or chronic, idiopathic enterocolitis. Our data suggest that SAA can serve as an effective noninvasive screening tool for amyloidosis and that minimally invasive biopsies can be used to confirm this diagnosis.
Authors:
Kelly A Rice; Edward S Chen; Kelly A Metcalf Pate; Eric K Hutchinson; Robert J Adams
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Comparative medicine     Volume:  63     ISSN:  1532-0820     ISO Abbreviation:  Comp. Med.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-06-13     Completed Date:  2014-01-15     Revised Date:  2014-06-02    
Medline Journal Info:
Nlm Unique ID:  100900466     Medline TA:  Comp Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  262-71     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amyloidosis / diagnosis*
Animals
Diagnosis, Differential
Enterocolitis / diagnosis*
Enzyme-Linked Immunosorbent Assay
Female
Macaca mulatta
Macaca nemestrina
Male
Serum Amyloid A Protein / metabolism
Grant Support
ID/Acronym/Agency:
P40 OD013117/OD/NIH HHS; P40-OD013117/OD/NIH HHS
Chemical
Reg. No./Substance:
0/Serum Amyloid A Protein
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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