| Diacylglycerol and triacylglycerol as responses in a dual response surface-optimized process for diacylglycerol production by lipase-catalyzed esterification in a pilot packed-bed enzyme reactor. | |
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MedLine Citation:
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PMID: 17571899 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Diacylglycerol (DAG) and triacylglycerol (TAG) as responses on optimization of DAG production using a dual response approach of response surface methodology were investigated. This approach takes the molecular equilibrium of DAG into account and allows for the optimization of reaction conditions to achieve maximum DAG and minimum TAG yields. The esterification reaction was optimized with four factors using a central composite rotatable design. The following optimized conditions yielded 48 wt % DAG and 14 wt % TAG: reaction temperature of 66.29 degrees C, enzyme dosage of 4 wt %, fatty acid/glycerol molar ratio of 2.14, and reaction time of 4.14 h. Similar results were achieved when the process was scaled up to a 10 kg production in a pilot packed-bed enzyme reactor. Lipozyme RM IM did not show any significant activity losses or changes in fatty acid selectivity on DAG synthesis during the 10 pilot productions. However, lipozyme RM IM displayed higher selectivity toward the production of oleic acid-enriched DAG. The purity of DAG oil after purification was 92 wt %. |
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Authors:
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Seong-Koon Lo; Ling-Zhi Cheong; Norlelawati Arifin; Chin-Ping Tan; Kamariah Long; Mohd Suria Affandi Yusoff; Oi-Ming Lai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-06-16 |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 55 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-07-03 Completed Date: 2007-09-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 5595-603 Citation Subset: IM |
Affiliation:
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Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Diglycerides
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biosynthesis*,
isolation & purification Enzymes, Immobilized Esterification Lipase / metabolism* Rhizomucor / enzymology Triglycerides / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Diglycerides; 0/Enzymes, Immobilized; 0/Triglycerides; EC 3.1.1.3/Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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