Document Detail

Diacylglycerol kinase zeta is associated with chromatin, but dissociates from condensed chromatin during mitotic phase in NIH3T3 cells.
MedLine Citation:
PMID:  18680142     Owner:  NLM     Status:  MEDLINE    
Diacylglycerol kinase (DGK) converts diacylglycerol (DG) to phosphatidic acid, both of which act as second messengers to mediate a variety of cellular mechanisms. Therefore, DGK contributes to the regulation of these messengers in cellular signal transduction. Of DGK isozymes cloned, DGKzeta is characterized by a nuclear localization signal that overlaps with a sequence similar to the myristoylated alanine-rich C-kinase substrate. Previous studies showed that nuclear DG is differentially regulated from plasma membrane DG and that the nuclear DG levels fluctuate in correlation with cell cycle progression, suggesting the importance of nuclear DG in cell cycle control. In this connection, DGKzeta has been shown to localize to the nucleus in fully differentiated cells, such as neurons and lung cells, although it remains elusive how DGK behaves during the cell cycle in proliferating cells. Here we demonstrate that DGKzeta localizes to the nucleus during interphase including G1, S, and G2 phases and is associated with chromatin although it dissociates from condensed chromatin during mitotic phase in NIH3T3 cells. Furthermore, this localization pattern is also observed in proliferating spermatogonia in the testis. These results suggest a reversible association of DGKzeta with histone or its related proteins in cell cycle, plausibly dependent on their post-translational modifications.
Hiroshi Hasegawa; Tomoyuki Nakano; Yasukazu Hozumi; Michiaki Takagi; Toshihiko Ogino; Masashi Okada; Ken Iseki; Hisatake Kondo; Masahiko Watanabe; Alberto M Martelli; Kaoru Goto
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  105     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-02     Completed Date:  2008-12-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  756-65     Citation Subset:  IM    
Copyright Information:
(c) 2008 Wiley-Liss, Inc.
Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Yamagata 990-9585, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Nucleus / metabolism
Chromatin / metabolism*
Chromatin Immunoprecipitation
Diacylglycerol Kinase / analysis,  metabolism*
Mice, Inbred C57BL
Mitosis / physiology*
NIH 3T3 Cells
Rats, Wistar
Reg. No./Substance:
0/Chromatin; EC Kinase; EC kinase zeta, mouse

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Spatial learning results in elevated agmatine levels in the rat brain.
Next Document:  Differential transactivation by orphan nuclear receptor NOR1 and its fusion gene product EWS/NOR1: p...