| Diacylglycerol and ceramide kinetics in primary cultures of activated T-lymphocytes. | |
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MedLine Citation:
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PMID: 8964608 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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T cell activation results in the generation of diacylglycerol (DAG), the physiological activator of protein kinase C. Recently, ceramide, a bioactive lipid intracellular second messenger, has been shown to play a positive role in T cell proliferation. Most studies examining mitogen induction of DAG and ceramide in T cells have been conducted in cell lines over short periods of time (0-30 min) relative to the 2-3-h time frame required for commitment to proliferation. Therefore, we examined T cell mitogen-induced DAG and ceramide kinetics under physiologically relevant conditions during the initial 2 h of culture. Freshly isolated murine splenic lymphocytes were stimulated with the T cell-specific mitogen, concanavalin A (Con A). Our results show that Con A induced a multiphasic DAG response with significant peaks in DAG mass occurring at 2, 20 and 120 min. Concomitantly, ceramide mass was significantly increased 2 min following Con A addition and remained elevated until 120 min. Addition of C8-ceramide (10 microM) to lymphocyte cultures significantly enhanced mitogen-induced proliferation. These results demonstrate that DAG is continuously produced by activated T lymphocytes in a multiphasic fashion, and that ceramide is a positive effector molecule with respect to murine T cell proliferation. These results establish a foundation for further examination of the relationship between DAG, ceramide and T cell activation. |
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Authors:
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C A Jolly; J C Laurenz; D N McMurray; R S Chapkin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Immunology letters Volume: 49 ISSN: 0165-2478 ISO Abbreviation: Immunol. Lett. Publication Date: 1996 Jan |
Date Detail:
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Created Date: 1996-12-17 Completed Date: 1996-12-17 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7910006 Medline TA: Immunol Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 43-8 Citation Subset: IM |
Affiliation:
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Faculty of Nutrition, Texas A and M University, College Station 77843, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Ceramides / metabolism*, pharmacology Concanavalin A / pharmacology* Diglycerides / metabolism*, pharmacology Female Kinetics Lymphocyte Activation / drug effects* Mice Mice, Inbred C57BL Signal Transduction / drug effects Spleen / cytology T-Lymphocytes / drug effects, immunology* |
| Chemical | |
Reg. No./Substance:
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0/Ceramides; 0/Diglycerides; 11028-71-0/Concanavalin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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