Document Detail


The diabetic ocular environment facilitates the development of endogenous bacterial endophthalmitis.
MedLine Citation:
PMID:  23036996     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: We tested the hypothesis that changes in the diabetic ocular environment facilitate the development of endogenous bacterial endophthalmitis (EBE).
METHODS: C57BL/6J mice were rendered diabetic with streptozotocin (STZ) for 1, 3, or 5 months' duration. Diabetic and age-matched nondiabetic mice were tail vein-injected with 10(8) CFU of Klebsiella pneumoniae, a common cause of EBE in diabetics. After either 2 or 4 days postinfection, the EBE incidence was assessed by electroretinography, histology, bacterial counts, and myeloperoxidase ELISAs. Blood-retinal barrier (BRB) permeability in uninfected diabetic mice also was determined.
RESULTS: No cases of EBE were observed among the 1-month diabetic group. Extending the time from diabetes induction to 3 months resulted in a 23.8% EBE incidence after 2 days, and a 22% incidence after 4 days. The incidence of EBE increased to 27% in the 5-month diabetic group. Infected eyes had an average 8.01 × 10(2) and 6.19 × 10(4) CFU/eye for the 3- and 5-month diabetic groups, respectively. There was no significant difference in BRB permeability between control and 1-month uninfected diabetic mice. However, 3- and 5-month diabetic mice had significantly greater BRB permeability than control mice. These results suggested that increasing the time from STZ diabetes induction to 3 and 5 months resulted in an ocular environment more conducive to the development of EBE.
CONCLUSIONS: These results demonstrated a correlation between an increase in BRB permeability and an increase in EBE incidence, supporting the hypothesis that diabetic ocular changes contribute to the development of EBE.
Authors:
Phillip S Coburn; Brandt J Wiskur; Elizabeth Christy; Michelle C Callegan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-11-01
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  53     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-05     Completed Date:  2013-01-15     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7426-31     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Capillary Permeability
Diabetes Mellitus, Experimental
Diabetic Retinopathy / complications*,  metabolism,  pathology
Disease Models, Animal
Electroretinography
Endophthalmitis / etiology,  microbiology*,  pathology
Eye Infections, Bacterial / etiology,  microbiology*,  pathology
Follow-Up Studies
Klebsiella Infections / etiology,  microbiology*,  pathology
Klebsiella pneumoniae / isolation & purification
Mice
Mice, Inbred C57BL
Retinal Vessels / metabolism,  pathology*
Grant Support
ID/Acronym/Agency:
P20RR17702/RR/NCRR NIH HHS; P30EY12191/EY/NEI NIH HHS; R01EY012985/EY/NEI NIH HHS
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