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Diabetes Mellitus Affects Biomechanical Properties of the Optic Nerve Head in the Rat.
MedLine Citation:
PMID:  22156545     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background: To investigate the effect of diabetes on the biomechanical behavior of the optic nerve head (ONH) and the peripapillary sclera (ppSc) in streptozocine-induced diabetic rats. Methods: Diabetes mellitus was induced in 20 Wistar rats using streptozocine. Twenty-five nondiabetic rats served as controls. Eyes were enucleated after 12 weeks and 2 strips of one eye were prepared containing ONH or ppSc. The stress-strain relation was measured in the stress range of 0.05-10 MPa using a biomaterial tester. Results: At 5% strain the stress of the ONH in diabetic rats was 897 ± 295 kPa and in the control group it was 671 ± 246 kPa; there was a significant difference between both groups (p = 0.011). The stress of the diabetic ppSc (574 ± 185 kPa) increased compared to that of the nondiabetic ppSc (477 ± 171 kPa), but this did not reach statistical significance (p = 0.174). The calculated tangent modulus at 5% strain was 11.79 MPa in the diabetic ONH and 8.77 MPa in the nondiabetic ONH; there was a significant difference between both groups (p = 0.006). The calculated tangent modulus at 5% strain was 7.17 MPa in the diabetic ppSc and 6.12 MPa in the nondiabetic ppSc, without a statistically significant difference (p = 0.09). Conclusion: In contrast to the ppSc, the ONH of diabetic rats showed a significant increase in stiffness compared to nondiabetic rats, which might be explained by nonenzymatic collagen cross-linking mediated by advanced glycation end products due to high blood glucose levels in diabetes. Further studies are needed to investigate if these biomechanical changes represent a detrimental risk factor for intraocular pressure regulation in diabetic glaucoma patients.
Authors:
Naim Terai; Eberhard Spoerl; Michael Haustein; Karin Hornykewycz; Janek Haentzschel; Lutz E Pillunat
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-07
Journal Detail:
Title:  Ophthalmic research     Volume:  47     ISSN:  1423-0259     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0267442     Medline TA:  Ophthalmic Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  189-194     Citation Subset:  -    
Copyright Information:
Copyright © 2011 S. Karger AG, Basel.
Affiliation:
Department of Ophthalmology, Carl Gustav Carus University Hospital, Dresden, Germany.
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