| DiO-C(18)-(3) Mediated Cell Fusion. | |
| | |
MedLine Citation:
|
PMID: 12219205 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
DiO, a fluorescent membrane probe, induced the rapid fusion of human erythrocytes and also mediated the fusion of mouse L-929 cells (fibroblastoma) in isotonic buffer (pH7.4) at room temperature. The fusion was independent of external Ca(2+) activating cysteine proteinase. PMSF, an inhibitor of serine proteinase, had no stopping effect on the fusion. The fluorescent cell images showed the existence of binding sites characteristic of high local concentration of DiO on the cell surface. Fluorescent filaments protruding from the cells and fused pairs were also recorded. These observations may have implication for a mechanism which promotes the protrusions of plasma membrane in the course of cell fusion. Based on the membrane electrostatics an the stress of the removal of water between bilayers, we infer that microregions of shared phospholipid bilayer may be formed at the distal ends of the protrusions when they are formed together as a consequence of the local dehydration action of the fusogen. |
| | |
Authors:
|
Cheng-Zhi Jin; Wei Li; Jiong Wang |
Related Documents
:
|
8789125 - Temporally resolved, independent stages of individual exocytotic secretion events. 12934825 - Incinerator slag formation and control of excursion temperatures. 16443655 - Low amounts of sucrose are sufficient to depress the phase transition temperature of dr... 11432735 - The physico-chemical characteristics of the phosphocholine-containing glycoglycerolipid... 15389865 - Linear ion traps in mass spectrometry. 20065095 - Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography. |
Publication Detail:
|
Type: JOURNAL ARTICLE |
Journal Detail:
|
Title: Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Volume: 29 ISSN: 0582-9879 ISO Abbreviation: Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Publication Date: 1997 |
Date Detail:
|
Created Date: 2002-Sep-9 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 20730160R Medline TA: Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) Country: - |
Other Details:
|
Languages: ENG Pagination: 273-279 Citation Subset: - |
Affiliation:
|
Laboratory of Cell Image Analysis, Research Center of Analysis Measurement, Fudan University, Shanghai 200433, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The Transferrins from Amphioxus and the Molecular Evolution of Transferrin.
Next Document: Studies on the Separation, Purification of Beijing Duck Apo A-I CNBr Cleavaged Fragments and the Loc...