Document Detail

Dexamethasone suppression of IL-1 beta-induced cyclooxygenase 2 expression is not mediated by lipocortin-1 in A549 cells.
MedLine Citation:
PMID:  8048967     Owner:  NLM     Status:  MEDLINE    
A549 cells grown under serum free conditions do not express cyclooxygenase 2 (cox 2). However, addition of IL-1 beta results in the induction of cox 2 in a time and dose dependent manner; actinomycin D completely blocks this induction. Dexamethasone completely suppressed the induction of cox 2 only if present during the first 3.5h of IL-1 beta treatment but not if added after 3.5h. Treatment with a neutralising monoclonal antibody (1A, Biogen) specific to lipocortin-1 failed to reverse this inhibition by dexamethasone. However, using this same antibody we were able to reverse completely the effects of dexamethasone on the suppression of PGE2 release. These observations support the idea that glucocorticoids directly regulate cox 2 expression at the transcriptional level and this phenomenon is not mediated by lipocortin-1.
S P Newman; R J Flower; J D Croxtall
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  202     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-09-01     Completed Date:  1994-09-01     Revised Date:  2010-08-25    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  931-9     Citation Subset:  IM    
Department of Biochemical Pharmacology, William Harvey Research Institute, Medical School of St. Batholomew's Hospital, London, UK.
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MeSH Terms
Annexins / immunology,  physiology
Antibodies, Monoclonal / pharmacology
Cell Line
Cycloheximide / pharmacology
Dactinomycin / pharmacology
Dexamethasone / pharmacology*
Dinoprostone / secretion
Epidermal Growth Factor / pharmacology
Gene Expression / drug effects*
Interleukin-1 / pharmacology*
Prostaglandin-Endoperoxide Synthases / biosynthesis,  genetics*
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Annexins; 0/Antibodies, Monoclonal; 0/Interleukin-1; 363-24-6/Dinoprostone; 50-02-2/Dexamethasone; 50-76-0/Dactinomycin; 62229-50-9/Epidermal Growth Factor; 66-81-9/Cycloheximide; EC Synthases

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