Document Detail


Dexamethasone suppresses mucus production and MUC-2 and MUC-5AC gene expression by NCI-H292 cells.
MedLine Citation:
PMID:  8843799     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Excessive production of airway mucus is a characteristic feature of many chronic inflammatory lung diseases. Although current pharmacological approaches to excessive mucus production are limited, glucocorticoids appear to be the most effective among a few useful drugs. The exact evidence for the effectiveness of glucocorticoids on mucus production has not been fully elucidated to date. The purpose of this study is to clarify the effect of dexamethasone on mucus production and mucin gene expression in a human pulmonary mucoepidermoid carcinoma cell line (NCI-H292). NCI-H292 cells produced hyaluronidase-resistant high-molecular-weight glycoconjugates (HMWG), which elute in the void volume on Sepharose CL-4B column chromatography. Dexamethasone significantly suppressed the basal production of [3H]glucosamine-or [3H]serine-labeled HMWG in NCI-H292 cells. In Northern blot analysis, dexamethasone attenuated steady-state mRNA levels of MUC-2 and MUC-5AC mucin genes. These data indicate that dexamethasone suppresses the basal production of HMWG and decreases steady-state mRNA levels of mucin genes in airway mucus-producing cancer cells.
Authors:
H Kai; K Yoshitake; A Hisatsune; T Kido; Y Isohama; K Takahama; T Miyata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  271     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1996-12-05     Completed Date:  1996-12-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  L484-8     Citation Subset:  IM    
Affiliation:
Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma / genetics,  metabolism
Dexamethasone / pharmacology*
Gene Expression Regulation, Neoplastic / drug effects*
Glucocorticoids / pharmacology*
Humans
Lung Neoplasms / genetics,  metabolism
Mucin-2
Mucins / biosynthesis*,  genetics
Neoplasm Proteins / biosynthesis,  genetics
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Glucocorticoids; 0/MUC2 protein, human; 0/Mucin-2; 0/Mucins; 0/Neoplasm Proteins; 50-02-2/Dexamethasone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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