Document Detail


Dexamethasone modulates osteogenesis and adipogenesis with regulation of osterix expression in rat calvaria-derived cells.
MedLine Citation:
PMID:  20717928     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Osteoblasts and adipocytes originate from common mesenchymal progenitor cells and although a number of compounds can induce osteoblastic and adipogenic differentiation from progenitor cells, the underlying mechanisms have not been elucidated. The present study examined the synergistic effects of dexamethasone (Dex) and bone morphogenetic protein (BMP)-2 on the differentiation of clonal mesenchymal progenitor cells isolated from rat calvaria into osteoblasts and adipocytes, as well as the effects of the timing of treatment. Cells were cultured for various periods of time in the presence of Dex and/or BMP-2. When cells were treated with Dex+BMP-2 during the early phase of differentiation, they differentiated into adipocytes. However, when cells were treated with Dex+BMP-2 during the late phase of differentiation, a synergistic effect on in vitro matrix mineralization was observed. To examine differences between the early and late phases of differentiation, ALP activity was measured in the presence of BMP-2. ALP activity increased markedly on Day 9, corresponding to the onset of the synergistic effect of Dex. Dex treatment inhibited osterix (OSX) expression in cells committed to adipogenic differentiation, but not in cells committed to osteogenic differentiation following BMP-2 treatment. The isoform2 OSX promoter region was found to be involved in the effects of Dex on cells during the early phase of differentiation. Furthermore, cells stably expressing OSX (isoform2) formed mineralized nodules even though they had been treated with Dex+BMP-2 during the early phase of differentiation. It appears that Dex modulates osteogenesis and adipogenesis in mesenchymal stem cells by regulating OSX expression.
Authors:
Yoshikazu Mikami; Mio Lee; Seiko Irie; Masaki J Honda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  226     ISSN:  1097-4652     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2010-12-30     Completed Date:  2011-01-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  739-48     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Wiley-Liss, Inc.
Affiliation:
Department of Anatomy, Nihon University School of Dentistry, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adipogenesis / drug effects*
Animals
Biological Markers / metabolism
Bone Morphogenetic Protein 2 / pharmacology
Calcification, Physiologic / drug effects
Cell Line
Dexamethasone / pharmacology*
Gene Expression Regulation / drug effects*
Humans
Lipids / chemistry
Osteogenesis / drug effects*
Promoter Regions, Genetic / genetics
RNA, Messenger / genetics,  metabolism
Rats
Skull / cytology*
Time Factors
Transcription Factors / genetics*,  metabolism
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Bone Morphogenetic Protein 2; 0/Lipids; 0/Osterix protein, rat; 0/RNA, Messenger; 0/Transcription Factors; 50-02-2/Dexamethasone

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