| Dexamethasone fails to inhibit the induction of cytosolic phospholipase A(2) expression by interleukin-1beta in cultured primary human amnion fibroblasts. | |
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MedLine Citation:
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PMID: 16338461 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the interaction of dexamethasone and interleukin-1beta (IL-1beta) on the expression of cytosolic phospholipase A(2) (cPLA(2)), the enzyme catalyzing the first reaction in the formation of prostaglandins, in cultured primary human amnion fibroblasts. DESIGN AND METHODS: Human amnion fibroblasts were prepared from fetal amnion collected at term and were treated with dexamethasone with or without interleukin-1beta for 24h. Prostaglandin E(2) (PGE(2)) output and cPLA(2) expression in cultured amnion fibroblasts were measured with radioimmunoassay, quantitative real-time polymerase chain reaction, Western blotting and cPLA(2) promoter-driven luciferase reporter gene activity. RESULTS: Both dexamethasone and IL-1beta caused a significant increase in prostaglandin E(2) output, cPLA(2) mRNA and protein expression in cultured human amnion fibroblasts. Both dexamethasone and IL-1beta stimulated cPLA(2) promoter-driven luciferase reporter gene activity. There was no obvious antagonistic or synergistic effect of combined treatment of dexamethasone and IL-1beta on PGE(2) output, cPLA(2) expression or cPLA(2) promoter-driven luciferase reporter gene activity in cultured human amnion fibroblasts. CONCLUSION: The above findings suggest that paradoxically dexamethasone is a stimulator for both prostaglandin synthesis and cPLA(2) expression in human amnion fibroblasts. The interaction between dexamethasone and IL-1beta on prostaglandin synthesis and cPLA(2) expression is neither synergistic nor conventionally antagonistic. |
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Authors:
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K Sun; X Qu; L Gao; L Myatt |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Placenta Volume: 27 ISSN: 0143-4004 ISO Abbreviation: Placenta Publication Date: 2006 Feb-Mar |
Date Detail:
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Created Date: 2005-12-12 Completed Date: 2006-03-23 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8006349 Medline TA: Placenta Country: England |
Other Details:
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Languages: eng Pagination: 164-70 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics, School of Life Sciences, Fudan University, Second Military Medical University, 220 Handan Road, Shanghai 200433, P.R. China. sunnygangsun@yahoo.com.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amnion
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cytology*,
drug effects*,
enzymology Cells, Cultured Cytosol / enzymology Dexamethasone / pharmacology* Dinoprostone / metabolism Female Fibroblasts / drug effects, enzymology Genes, Reporter Humans Interleukin-1 / pharmacology* Luciferases / analysis, genetics Phospholipases A / antagonists & inhibitors, genetics, metabolism* Promoter Regions, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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HD31514/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-1; 363-24-6/Dinoprostone; 50-02-2/Dexamethasone; EC 1.13.12.-/Luciferases; EC 3.1.1.-/Phospholipases A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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