Document Detail


Dexamethasone effects on myoblast proliferation and differentiation.
MedLine Citation:
PMID:  7188899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Effects of glucocorticoids on myoblast proliferation and differentiation were determined in cell culture. Dexamethasone (1 X 10(-7) M) decreased myoblast doubling time by 7 h and increased cell densities as much as 800% after 8 days of exposure of the cells to dexamethasone. Stimulation of myoblast proliferation by glucocorticoids was dose dependent, with half-maximal activity exhibited at concentrations of 3 X 10(-9) M dexamethasone or 2.3 X 10(-8) M corticosterone. In differentiating cultures, the rate of accumulation and final levels of DNA and creatine phosphokinase were highly stimulated by dexamethasone. However, normalization of creatine phosphokinase levels (a marker for myoblast differentiation) per microgram DNA demonstrated a small (30%) decrease in differentiation in cultures exposed to glucocorticoids. These results indicate that glucocorticoids stimulate early myogenesis by increasing the number of myoblasts but do not stimulate the differentiation of these cells.
Authors:
V Guerriero; J R Florini
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  106     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1980 Apr 
Date Detail:
Created Date:  1980-05-30     Completed Date:  1980-05-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1198-202     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Cell Differentiation / drug effects
Cell Division / drug effects
Chick Embryo
Creatine Kinase / metabolism
DNA / metabolism
Dexamethasone / pharmacology*
Dose-Response Relationship, Drug
Kinetics
Muscles / drug effects,  metabolism,  physiology*
Rats
Chemical
Reg. No./Substance:
50-02-2/Dexamethasone; 9007-49-2/DNA; EC 2.7.3.2/Creatine Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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