| Dexamethasone effects on myoblast proliferation and differentiation. | |
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MedLine Citation:
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PMID: 7188899 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Effects of glucocorticoids on myoblast proliferation and differentiation were determined in cell culture. Dexamethasone (1 X 10(-7) M) decreased myoblast doubling time by 7 h and increased cell densities as much as 800% after 8 days of exposure of the cells to dexamethasone. Stimulation of myoblast proliferation by glucocorticoids was dose dependent, with half-maximal activity exhibited at concentrations of 3 X 10(-9) M dexamethasone or 2.3 X 10(-8) M corticosterone. In differentiating cultures, the rate of accumulation and final levels of DNA and creatine phosphokinase were highly stimulated by dexamethasone. However, normalization of creatine phosphokinase levels (a marker for myoblast differentiation) per microgram DNA demonstrated a small (30%) decrease in differentiation in cultures exposed to glucocorticoids. These results indicate that glucocorticoids stimulate early myogenesis by increasing the number of myoblasts but do not stimulate the differentiation of these cells. |
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Authors:
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V Guerriero; J R Florini |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Endocrinology Volume: 106 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1980 Apr |
Date Detail:
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Created Date: 1980-05-30 Completed Date: 1980-05-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1198-202 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Cell Differentiation / drug effects Cell Division / drug effects Chick Embryo Creatine Kinase / metabolism DNA / metabolism Dexamethasone / pharmacology* Dose-Response Relationship, Drug Kinetics Muscles / drug effects, metabolism, physiology* Rats |
| Chemical | |
Reg. No./Substance:
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50-02-2/Dexamethasone; 9007-49-2/DNA; EC 2.7.3.2/Creatine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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