Document Detail

Dexamethasone alleviates meconium-induced airway hyperresponsiveness and lung inflammation in rabbits.
MedLine Citation:
PMID:  16229002     Owner:  NLM     Status:  MEDLINE    
The effects of dexamethasone on in vitro airway reactivity associated with lung inflammation were investigated in rabbits with meconium aspiration. Oxygen-ventilated adult rabbits received an intratracheal bolus of 4 ml/kg body weight of saline (Sal, n = 4) or human meconium (25 mg/ml). Thirty minutes later, meconium-instilled animals intravenously received 0.5 mg/kg of dexamethasone (Dexa, n = 6), or were left without treatment (Meco, n = 5). The animals were ventilated for a further 5 hr and then sacrificed. The left lungs were lavaged with saline, and the white blood cell (WBC) count was estimated. Tracheal and right-lung tissue strips were placed into organ chambers with Krebs-Henseleit solution. Cumulative doses of histamine (10(-8)-10(-3) mol/l) and acetylcholine (10(-8)-10(-3) mol/l) were added to the chambers, and recordings of contractions were made after a 30-min loading phase with a tension of 4 grams, and another 30-min adaptation phase with a tension of 2 g. Tracheal smooth muscle in vitro reactivity to histamine was higher in the Meco than in the Sal group, and dexamethasone decreased the reactivity compared to the Meco group (P < 0.05). Lung tissue in vitro reactivity to histamine was slightly higher in the Meco than in the Sal group (P > 0.05), and dexamethasone decreased the reactivity compared to both the Meco and Sal groups (P < 0.05). No between-group differences were observed in tracheal or lung in vitro reactivity to acetylcholine (P > 0.05). In the Meco group, blood WBC (P > 0.05) and neutrophil (P < 0.05) counts were lower than in the Sal and Dexa groups. Lung neutrophils and eosinophils were higher in both the Meco and Dexa groups than in the Sal group (P < 0.01). Dexamethasone decreased neutrophils (P < 0.05) compared to the Meco group. Meconium-induced airway hyperreactivity to histamine and lung inflammation were alleviated by dexamethasone.
Juraj Mokry; Daniela Mokra; Martina Antosova; Janka Bulikova; Andrea Calkovska; Gabriela Nosalova
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pediatric pulmonology     Volume:  41     ISSN:  8755-6863     ISO Abbreviation:  Pediatr. Pulmonol.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-15     Completed Date:  2007-05-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510590     Medline TA:  Pediatr Pulmonol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  55-60     Citation Subset:  IM    
Copyright Information:
Copyright 2005 Wiley-Liss, Inc.
Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia.
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MeSH Terms
Anti-Inflammatory Agents / administration & dosage,  pharmacology*
Dexamethasone / administration & dosage,  pharmacology*
Disease Models, Animal
Infant, Newborn
Lung / drug effects*,  pathology,  physiopathology
Meconium / chemistry*
Meconium Aspiration Syndrome / physiopathology
Muscle, Smooth / drug effects
Pneumonia / drug therapy*
Trachea / drug effects*,  pathology,  physiopathology
Treatment Outcome
Reg. No./Substance:
0/Anti-Inflammatory Agents; 50-02-2/Dexamethasone

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