Document Detail

Dexamethasone affects cytokine-mediated adhesion of HL-60 human promyelocytic leukemia cells to cultured dermal microvascular endothelial cells.
MedLine Citation:
PMID:  11358436     Owner:  NLM     Status:  MEDLINE    
Leukocyte endothelial adhesion (LEA) is the prelude to a complex cascade of reactions following an immunological challenge. Recently, LEA has been implicated in the molecular basis of several dermatological disorders. While the role of proinflammatory cytokines, such as interleukin-1 beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha), in LEA has been investigated using nondermal models, limited data exist regarding their effects on LEA in dermal models. This study shows that cotreatment of cultured human dermal endothelial cells (CADMEC) with IL-1beta and TNF-alpha resulted in a marked increase in the adherence of human promyelocytic leukemia (HL-60) cells to CADMEC and an increase in expression of intercellular adhesion molecule-1 and E-selectin. Pretreatment of CADMEC with dexamethasone, a long-lasting glucocorticoid, resulted in a decrease in both HL-60 cell adhesion to CADMEC and adhesion molecule expression. Taken together, these data demonstrate that LEA may play a role in inflammatory skin conditions and in the mechanisms underlying the potential use of glucocorticoids as a treatment option.
N A Patel; J A Patel; M F Stins; K S Kim; S L Chang
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinical immunology (Orlando, Fla.)     Volume:  99     ISSN:  1521-6616     ISO Abbreviation:  Clin. Immunol.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-05-18     Completed Date:  2001-06-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100883537     Medline TA:  Clin Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  387-94     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Department of Biology, Seton Hall University, South Orange, New Jersey 07079, USA.
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MeSH Terms
Cell Adhesion / drug effects
Cells, Cultured
Dexamethasone / pharmacology*
Dose-Response Relationship, Drug
E-Selectin / analysis
Endothelium, Vascular / cytology*
HL-60 Cells
Intercellular Adhesion Molecule-1 / analysis
Interleukin-1 / pharmacology*
Leukocytes / physiology
Skin / blood supply,  immunology*
Tumor Necrosis Factor-alpha / pharmacology*
Vascular Cell Adhesion Molecule-1 / analysis
Grant Support
Reg. No./Substance:
0/E-Selectin; 0/Interleukin-1; 0/Tumor Necrosis Factor-alpha; 0/Vascular Cell Adhesion Molecule-1; 126547-89-5/Intercellular Adhesion Molecule-1; 50-02-2/Dexamethasone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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