Document Detail


Dexamethasone-FITC staining application for measurement of circadian rhythmicity of glucocorticoid receptor expression in mouse living thymocyte subsets.
MedLine Citation:
PMID:  23726907     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Glucocorticoids are involved in the regulation of immune homeostasis and thymopoiesis and the integration of the thymus function with the neuroendocrine system. Their regulatory function is closely related to glucocorticoid receptor (GCR) expression. The aim of this study was to develop a method for the measurement of GCR expression in mouse living thymocytes by flow cytometry. Using dexamethasone binding we have shown differences in GCR expression among thymocyte subsets and their dependence on the circadian rhythm.
Authors:
Anna Kowalik; Ewelina Kiernozek; Mariusz Kulinczak; Klaudia Brodaczewska; Ewa Kozlowska; Katarzyna Gieczewska; Carlo Riccardi; Nadzieja Drela
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-5-30
Journal Detail:
Title:  Journal of neuroimmunology     Volume:  -     ISSN:  1872-8421     ISO Abbreviation:  J. Neuroimmunol.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-6-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8109498     Medline TA:  J Neuroimmunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Elsevier B.V. All rights reserved.
Affiliation:
Department of Immunology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
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