Document Detail

Developmentally retarded frozen blastocysts can be rescued by synchronizing culture prior to transfer.
MedLine Citation:
PMID:  16790109     Owner:  NLM     Status:  MEDLINE    
Asynchrony between embryo development and endometrial differentiation is the limiting step of successful pregnancy in assisted reproduction. The aim of this study was to investigate whether or not post-thaw synchronization culture of day 5-6 frozen embryos, prior to transfer, with endometrial differentiation resulted in pregnancy. A total of 142 cycles of 134 patients were transferred in three protocols. Blastocysts with cavities larger than half of the entire blastocyst volume were transferred without synchronizing culture on day 5 or 6 of progesterone commencement (P5/6) in hormone replacement treatment cycles (protocol 1). Blastocysts with cavitation below half of the entire blastocyst were cultured for 1 or 2 days after thawing prior to transfer on P5 or P6 (protocol 2). Morulae and very early stage blastocysts were thawed on the days corresponding to P5 and P6, and only the embryos that reached expanded or hatching blastocysts were transferred on P7 without synchronizing culture (protocol 3). Pregnancy rate in protocol 2 (32.0%) was comparable with that of protocol 1 (35.0%). It is concluded that developmentally retarded frozen embryos can be rescued with synchronizing culture prior to transfer by evading asynchrony.
Takafumi Utsunomiya; Hiroko Ito; Kaori Hirai; Eiko Otsu; Hirohiko Watanabe; Takahide Mori
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Publication Detail:
Type:  Clinical Trial; Journal Article    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  12     ISSN:  1472-6483     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-06-22     Completed Date:  2006-07-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  622-9     Citation Subset:  IM    
St Luke Clinic, Oita, Japan.
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MeSH Terms
Blastocyst / drug effects,  pathology,  physiology*
Embryo Culture Techniques*
Embryo Implantation
Embryo Transfer*
Embryonic Development*
Pregnancy Rate
Progesterone / pharmacology
Tissue Preservation / methods
Reg. No./Substance:

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