Document Detail


Developmentally regulated cell cycle dependence of swelling-activated anion channel activity in the mouse embryo.
MedLine Citation:
PMID:  11566849     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Anion channels activated by increased cell volume are a nearly ubiquitous mechanism of cell volume regulation, including in early preimplantation mouse embryos. Here, we show that the swelling-activated anion current (I(Cl,swell)) in early mouse embryos is cell-cycle dependent, and also that this dependence is developmentally regulated. I(Cl,swell) is present both in first meiotic prophase (germinal vesicle stage) mouse oocytes and in unfertilized mature oocytes in second meiotic metaphase, and it persists after fertilization though the 1-cell and 2-cell stages. I(Cl,swell) was found to remain unchanged during metaphase at the end of the 1-cell stage. However, I(Cl,swell) decreased during prophase and became nearly undetectable upon entry into metaphase at the end of the 2-cell stage. Entry into prophase/metaphase was required for the decrease in I(Cl,swell) at the end of the 2-cell stage, since it persisted indefinitely in 2-cell embryos arrested in late G(2). There is considerable evidence that the channel underlying I(Cl,swell) is not only permeable to inorganic anions, but to organic osmolytes as well. We found a similar pattern of cell cycle and developmental dependence in the 1-cell and 2-cell stages for the swelling-induced increase in permeability to the organic osmolyte glycine. Thus, entry into metaphase deactivates I(Cl,swell) in embryos, but only after developmental progression through the 2-cell stage.
Authors:
M Kolajova; M A Hammer; J L Collins; J M Baltz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  128     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-09-21     Completed Date:  2001-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  3427-34     Citation Subset:  IM    
Affiliation:
Hormones, Growth and Development Unit, Ottawa Health Research Institute, University of Ottawa, Ottawa, Ontario, K1Y 4E9 Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anions / metabolism*
Blastocyst / cytology*,  physiology*
Cell Cycle
Cell Membrane Permeability
Cell Size
Electric Conductivity
Glycine / metabolism
Ion Channels / metabolism*
Metaphase
Mice
Chemical
Reg. No./Substance:
0/Anions; 0/Ion Channels; 56-40-6/Glycine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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