Document Detail


Developmentally regulated Gb3 galactosyltransferase and alpha-galactosidase determine Shiga toxin receptors in intestine.
MedLine Citation:
PMID:  7943326     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The receptor for Shiga toxin on rabbit intestinal microvillus membranes (MVMs) has been identified as a developmentally regulated glycolipid, globotriaosylceramide [galactose alpha 1-4 galactose beta 1-4 glucose beta 1-1 ceramide (Gb3)]. MVM Gb3 levels increase markedly in the third week of life, concomitant with fluid secretory responses to the toxin. To study mechanisms controlling developmental regulation of MVM Gb3, we measured the specific synthetic Gb3 galactosyltransferase and degradative alpha-galactosidase activities and subcellular distribution of Gb3 at various ages. Quantitative high-performance liquid chromatography demonstrated a similar developmental pattern in both microsomal and MVM Gb3, indicating that late expression of MVM Gb3 is not due to delayed migration of Gb3 from the microsomal to the MVM. The specific Gb3 galactosyltransferase activity increased with age, with a sharp increase seen at 18 days of age, whereas alpha-galactosidase activity followed an inverse pattern. Thus, regulation of both synthetic and degradative pathways for Gb3 appears to explain the observed changes in Gb3 levels with age. The nature of the signals for developmental regulation of Gb3 levels is unknown, as are the physiological consequences of altered MVM glycolipid composition other than mediating response to Shiga toxin.
Authors:
M Mobassaleh; O Koul; K Mishra; R H McCluer; G T Keusch
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  267     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1994-11-22     Completed Date:  1994-11-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  G618-24     Citation Subset:  IM    
Affiliation:
Division of Pediatric Gastroenterology and Nutrition, New England Medical Center, Boston.
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MeSH Terms
Descriptor/Qualifier:
Aging / metabolism
Animals
Animals, Newborn
Galactosyltransferases / chemistry,  metabolism*
Glycolipids / metabolism
Intestine, Small / enzymology*
Kinetics
Lipid Metabolism
Rabbits
Receptors, Cell Surface / metabolism
Subcellular Fractions / metabolism
Tissue Distribution
Trihexosylceramides / metabolism*
alpha-Galactosidase / metabolism*
Grant Support
ID/Acronym/Agency:
AI-16242/AI/NIAID NIH HHS; DK-34928/DK/NIDDK NIH HHS; DK-39120/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Glycolipids; 0/Receptors, Cell Surface; 0/Trihexosylceramides; 71965-57-6/globotriaosylceramide; EC 2.4.1.-/Galactosyltransferases; EC 2.4.1.-/UDP-galactose-lactosylceramide alpha 1-4-galactosyltransferase; EC 3.2.1.22/alpha-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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