Document Detail

Developmentally controlled transcription of HLA and beta 2-microglobulin genes in human teratocarcinoma lines.
MedLine Citation:
PMID:  6187267     Owner:  NLM     Status:  MEDLINE    
Poly(A+) RNA have been extracted from the human adult cell lines Raji and D98 (HeLa) and from the teratocarcinoma-derived cell lines TeraI and PA1. The TeraI cell line expresses many markers in common with undifferentiated mouse embryonal carcinoma cells and has been previously shown to be less differentiated than PA1. Only small amounts of poly(A+) RNA extracted from TeraI were found to hybridize with human HLA and beta 2-microglobulin (beta 2m) cDNA probes, while poly(A+) RNA from PA1 and the two other adult cell lines exhibited intense hybridization to these two probes. Our results correlate with the differentiation status of the various cell lines as defined by serological and biochemical analysis and suggest that HLA and beta 2m gene expression is mainly controlled at the transcriptional level.
D Morello; P Avner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annales d'immunologie     Volume:  133D     ISSN:  0300-4910     ISO Abbreviation:  Ann. Immunol. (Paris)     Publication Date:    1982 Sep-Oct
Date Detail:
Created Date:  1983-04-15     Completed Date:  1983-04-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0353045     Medline TA:  Ann Immunol (Paris)     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  121-9     Citation Subset:  IM    
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MeSH Terms
Antibodies, Monoclonal / immunology
Beta-Globulins / genetics*
Cell Differentiation
Cell Line
HLA Antigens / genetics*
Poly A / genetics
RNA, Messenger / genetics
Teratoma / genetics*,  immunology
Transcription, Genetic*
beta 2-Microglobulin / genetics*,  immunology
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Beta-Globulins; 0/HLA Antigens; 0/RNA, Messenger; 0/beta 2-Microglobulin; 24937-83-5/Poly A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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