| Developmental and topographic expression of neutral glycosphingolipids in vertebrate brain. | |
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MedLine Citation:
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PMID: 9343945 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have examined neutral glycosphingolipid (Ngsl) expression in embryonic (E), post-natal (P) and adult rodent brain employing digoxigenin immunostaining (DIG-IS) and anti-Ngsl antisera (both monospecific polyclonal and monoclonal) directed toward specific carbohydrates. Several previously unknown long-chain (-CHO = or > 4) Ngsls have been identified. Four Ngsls have been purified and characterized as GgOse4Cer or GA1, Galactosyl beta 1-3globoside, Fuc alpha 1-3nLcOse4Cer or Lewis X (Le(x)) and a novel GalNAc beta 1-4GA1. A few transient bands appear at different developmental ages. Several fast migrating cerebrosides have also been identified during the early phase of active myelination and tentatively characterized as derivatives of galactosylceramide. Immunohistochemical localization of GA1, Le(x) and GalNAc-GA1 in adult rodent brain shows unique and specific cellular topographies of these carbohydrate antigens. |
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Authors:
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S Dasgupta; E L Hogan |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Indian journal of biochemistry & biophysics Volume: 34 ISSN: 0301-1208 ISO Abbreviation: Indian J. Biochem. Biophys. Publication Date: 1997 Feb-Apr |
Date Detail:
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Created Date: 1997-12-23 Completed Date: 1997-12-23 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0310774 Medline TA: Indian J Biochem Biophys Country: INDIA |
Other Details:
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Languages: eng Pagination: 166-9 Citation Subset: IM |
Affiliation:
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Department of Neurology, Medical University of South Carolina, Charleston 29425, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / embryology, growth & development, metabolism* Carbohydrate Sequence Glycosphingolipids / chemistry, metabolism* Immunohistochemistry Molecular Sequence Data Molecular Structure Rats |
| Grant Support | |
ID/Acronym/Agency:
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NS 31355/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Glycosphingolipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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