Document Detail


Developmental switch of S-layer protein synthesis in Bacillus anthracis.
MedLine Citation:
PMID:  11952909     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adjustment of the synthesis of abundant protein to the requirements of the cell involves processes critical to the minimization of energy expenditure. The regulation of S-layer genes might be a good model for such processes because expression must be controlled, such that the encoded proteins exactly cover the surface of the bacterium. Bacillus anthracis has two S-layer genes, sap and eag, encoding the S-layer proteins Sap and EA1 respectively. We report that the production and surface localization of Sap and EA1 are under developmental control, suggesting that an exponential phase 'Sap layer' is subsequently replaced by a stationary phase 'EA1 layer'. This switch is controlled at the transcriptional level: sap is most certainly transcribed by RNA polymerase containing sigmaA, whereas eag expression depends on sigmaH. More importantly, Sap is required for the temporal control of eag, and EA1 is involved in strict feedback regulation of eag. This control may be direct because both S-layer proteins bind, in vitro, the eag promoter, specifically suggesting that they might act as transcriptional repressors.
Authors:
Tâm Mignot; Stéphane Mesnage; Evelyne Couture-Tosi; Michèle Mock; Agnès Fouet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular microbiology     Volume:  43     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-04-15     Completed Date:  2002-08-02     Revised Date:  2008-04-24    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1615-27     Citation Subset:  IM    
Affiliation:
Toxines et Pathogénie Bactériennes (URA 2172, CNRS), Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Bacillus anthracis / genetics,  growth & development*,  metabolism
Bacterial Proteins / biosynthesis*,  genetics
Base Sequence
Gene Expression Regulation, Bacterial*
Membrane Glycoproteins / biosynthesis*,  genetics
Microscopy, Electron
Molecular Sequence Data
Sigma Factor / metabolism
Transcription, Genetic*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Membrane Glycoproteins; 0/S-layer proteins; 0/Sigma Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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