Document Detail


Developmental regulation of genomic imprinting during gametogenesis.
MedLine Citation:
PMID:  7589806     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Successful mammalian development requires both the male and female genomes. This is due in part to genomic imprinting, which results in offspring inheriting only one functional copy of a gene from either the mother or the father. Evidence suggests that this specialization of the parental genomes is established during gametogenesis when the imprint pattern inherited from the parent is switched to reflect the sex of the progeny. We used reverse transcription-PCR to analyze the allele-specific expression of Igf-2, Igf-2r, and H19 in the testes and ovaries of mice derived from an interspecies cross between Mus musculus and Mus spretus. Because of genomic imprinting, Igf-2 is expressed only from the paternal allele and Igf-2r and H19 only from the maternal allele, in most tissues. Although allele-specific expression was maintained in the neonatal testis and ovary, relaxation of imprinting was detected by 7 days after birth in the male and continued during testis development. In the female, relaxation of the Igf-2 and Igf-2r parental imprints was observed in the adult ovary and oocyte. These results (1) indicate that imprinted expression is relaxed during gametogenesis, presumably as a consequence or prerequisite of the imprinting mechanism, and (2) predict a subsequent imprinting event after which the allele-specific expression of Igf-2, Igf-2r, and H19 reflects the parent of origin.
Authors:
A J Villar; E M Eddy; R A Pedersen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental biology     Volume:  172     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1995-12-26     Completed Date:  1995-12-26     Revised Date:  2011-10-07    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  264-71     Citation Subset:  IM    
Affiliation:
Department of Anatomy, University of California, San Francisco 94143, USA.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Animals
Animals, Newborn
Base Sequence
Crosses, Genetic
DNA Primers
Embryonic and Fetal Development / genetics*
Female
Gene Expression Regulation*
Genomic Imprinting*
Insulin-Like Growth Factor II / biosynthesis,  genetics
Male
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Muridae / genetics
Muscle Proteins / biosynthesis,  genetics
Oocytes / metabolism
Organ Specificity
Ovary / metabolism
Phenotype
Polymerase Chain Reaction
RNA, Untranslated*
Receptor, IGF Type 2 / biosynthesis,  genetics
Testis / metabolism
Tongue / metabolism
Grant Support
ID/Acronym/Agency:
HD26732/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/H19 long non-coding RNA; 0/Muscle Proteins; 0/RNA, Untranslated; 0/Receptor, IGF Type 2; 67763-97-7/Insulin-Like Growth Factor II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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